TY - JOUR
AU1 - Ozaki, Nobuaki
AU2 - Shibasaki, Tadao
AU3 - Kashima, Yasushige
AU4 - Miki, Takashi
AU5 - Takahashi, Kazuo
AU6 - Ueno, Hiroaki
AU7 - Sunaga, Yasuhiro
AU8 - Yano, Hideki
AU9 - Matsuura, Yoshiharu
AU1 - Iwanaga, Toshihiko
AU1 - Takai, Yoshimi
AU1 - Seino, Susumu
AB - Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, Rim2, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis.
TI - cAMP-GEFII is a direct target of cAMP in regulated exocytosis
JF - Nature Cell Biology
DO - 10.1038/35041046
DA - 2000-10-10
UR - https://www.deepdyve.com/lp/springer-journals/camp-gefii-is-a-direct-target-of-camp-in-regulated-exocytosis-V1s8ehuLRz
SP - 805
EP - 811
VL - 2
IS - 11
DP - DeepDyve
ER -