TY - JOUR AU1 - Ozaki, Nobuaki AU2 - Shibasaki, Tadao AU3 - Kashima, Yasushige AU4 - Miki, Takashi AU5 - Takahashi, Kazuo AU6 - Ueno, Hiroaki AU7 - Sunaga, Yasuhiro AU8 - Yano, Hideki AU9 - Matsuura, Yoshiharu AU1 - Iwanaga, Toshihiko AU1 - Takai, Yoshimi AU1 - Seino, Susumu AB - Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, Rim2, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis. TI - cAMP-GEFII is a direct target of cAMP in regulated exocytosis JF - Nature Cell Biology DO - 10.1038/35041046 DA - 2000-10-10 UR - https://www.deepdyve.com/lp/springer-journals/camp-gefii-is-a-direct-target-of-camp-in-regulated-exocytosis-V1s8ehuLRz SP - 805 EP - 811 VL - 2 IS - 11 DP - DeepDyve ER -