TY - JOUR
AU - Kim, Jin-Soo
AB - Summary: The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a targeted manner. CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limited by off-target mutations. Here, we present a novel algorithm termed Cas-OFFinder that searches for potential off-target sites in a given genome or user-defined sequences. Unlike other algorithms currently available for identification of RGEN off-target sites, Cas-OFFinder is not limited by the number of mismatches and allows variations in protospacer-adjacent motif sequences recognized by Cas9, the essential protein component in RGENs. Cas-OFFinder is available as a command-line program or accessible via our website.Availability and implementation: Cas-OFFinder free access at http://www.rgenome.net/cas-offinder.Contact:
baesau@snu.ac.kr or jskim01@snu.ac.kr
TI - Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
JF - Bioinformatics
DO - 10.1093/bioinformatics/btu048
DA - 2014-01-24
UR - https://www.deepdyve.com/lp/pubmed-central/cas-offinder-a-fast-and-versatile-algorithm-that-searches-for-W95rLQcrt5
SP - 1473
EP - 1475
VL - 30
IS - 10
DP - DeepDyve
ER -