TY - JOUR
AU - Bhatia, Sangeeta N.
AB - The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called “CRISPR‐plus” (CRISPR‐precise light‐mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR‐plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.
TI - Development of Light‐Activated CRISPR Using Guide RNAs with Photocleavable Protectors
JF - Angewandte Chemie International Edition
DO - 10.1002/anie.201606123
DA - 2016-09-26
UR - https://www.deepdyve.com/lp/wiley/development-of-light-activated-crispr-using-guide-rnas-with-a306mxL0Os
SP - 12440
EP - 12444
VL - 55
IS - 40
DP - DeepDyve
ER -