TY - JOUR
AU - Dean, Caroline
AB - Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.
TI - Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing.
JF - Science
DO - 10.1126/science.1180278
DA - 2010-01-25
UR - https://www.deepdyve.com/lp/pubmed/targeted-3-processing-of-antisense-transcripts-triggers-arabidopsis-b9gnUSCeE1
SP - 94
EP - 7947
VL - 327
IS - 5961
DP - DeepDyve
ER -