TY - JOUR AU - Hagen, C. W. AB - Abstract Extracts of the flower petals of Impatiens balsamina L. contain enzymes which catalyze the glycosylation of phenolic compounds. Enzymes have been extracted which glycosylate hydroquinone to arbutin and at least 3 different flavonols to the 3-monoglucoside. The hydroquinone glucosylating enzyme is similar to enzymes previously described except that it requires an unidentified low molecular weight cofactor. The glucosylation of flavonols follows normal enzyme kinetics; it requires a nucleotide diphosphate glucose donor for activity, and is made more evident by the presence of glucono-1:5-lactone, an inhibitor of endogenous glucosidases. It is suggested that the flavonol glucosylating enzyme acts naturally to glucosylate a precursor of both flavonols and anthocyanins to the 3-monoglucoside. The only elaboration of an anthocyanin observed with petal extracts was an acylation of pelargonidin-3-monoglucoside. 3 Present address: Section of Genetics, Development and Physiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14850. 1 This paper has been dedicated to Professor Ralph E. Cleland on the occasion of his seventy-fifth birthday in recognition of the stimulation and academic guidance which he has provided for both authors. 2 The work was supported in part by National Science Foundation Grants G21943, GB1978 and GB5065 (CWH) and by a Public Health Service Predoctoral Fellowship GM032, 599-01 (CDM). This content is only available as a PDF. © 1968 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) TI - The Differentiation of Pigmentation in Flower Parts, IV. Flavonoid Elaborating Enzymes From Petals of Impatiens balsamina s JF - Plant Physiology DO - 10.1104/pp.43.9.1347 DA - 1968-09-01 UR - https://www.deepdyve.com/lp/oxford-university-press/the-differentiation-of-pigmentation-in-flower-parts-iv-flavonoid-cC2X0Zg0el SP - 1347 EP - 1354 VL - 43 IS - 9 DP - DeepDyve ER -