TY - JOUR
AU - Stuart, K
AB - In the mitochondria of trypanosomatid protozoa the precursors of messenger RNAs (pre-mRNAs) have their coding information remodeled by the site-specific insertion and deletion of uridylate (U) residues. Small trans-acting guide RNAs (gRNAs) supply the genetic information for this RNA editing. An in vitro system was developed to study the mechanism of U insertion into pre-mRNA. U-insertion editing occurs through a series of enzymatic steps that begin with gRNA-directed pre-mRNA cleavage. Inserted U's are derived from free uridine triphosphate and are added to the 3' terminus of a 5' pre-mRNA cleavage product. gRNA specifies edited RNA sequence at the subsequent ligation step by base pairing-mediated juxtaposition of the 3' cleavage product and the processed 5' cleavage product. gRNA/pre-mRNA chimeras, purported intermediates, seem to be abortive end products of the same reaction.
TI - RNA editing: a mechanism for gRNA-specified uridylate insertion into precursor mRNA.
JF - Science
DO - 10.1126/science.273.5279.1189
DA - 1996-09-24
UR - https://www.deepdyve.com/lp/pubmed/rna-editing-a-mechanism-for-grna-specified-uridylate-insertion-into-cnjdE6z22G
SP - 1189
EP - 95
VL - 273
IS - 5279
DP - DeepDyve
ER -