TY - JOUR
AU - Arif, Mohammad
AB - Bacteria have developed several defense mechanisms againstbacteriophages over evolutionary time, but the concept of prokaryotic RNAinterference mediated defense mechanism against phages and other invadinggenetic elements has emerged only recently. Clustered regularly interspacedshort palindromic repeats (CRISPR) together with closely associated genes (cas genes)constitute the CASS system that is believed to provide a RNAi-likedefense mechanism against bacteriophages within the host bacterium. However, aCASS mediated RNAi-like pathway in enteric pathogens such as Vibriocholerae O395 or Escherichia coli O157 have not been reported yet. This study specifically was designed to investigate the possibility and evolutionaryorigin of CASS mediated RNAi-like pathway in the genome of a set of entericpathogens, especially V. cholerae. The results showed that V.cholerae O395 and also other related enteric pathogens have the essential CASS components (CRISPR and cas genes) to mediate a RNAi-like pathway. Thefunctional domains of a V. cholerae Cas3 protein, which is believed toact as a prokaryotic Dicer, was revealed and compared with the domains ofeukaryotic Dicer proteins. Extensive homology in several functional domainsprovides significant evidence that the Cas3 protein has the essential domainsto play a vital role in RNAi like pathway in V. cholerae. The secondarystructure of the pre-siRNA for V. cholerae O395 was determined and itsthermodynamic stability also reinforced the previous findings and signifies theprobability of a RNAi-like pathway in V. cholerae O395.
TI - Assessment of the Evolutionary Origin and Possibility of			 CRISPR-Cas (CASS) Interference Pathway in Vibrio cholerae O395
JF - In Silico Biology
DO - 10.3233/isb-2009-0402
DA - 2009-08-01
UR - https://www.deepdyve.com/lp/ios-press/assessment-of-the-evolutionary-origin-and-possibility-of-crispr-cas-e39y0jUi7t
SP - 245
EP - 254
VL - 9
IS - 4
DP - DeepDyve
ER -