TY - JOUR
AU1 - Miyata, Tomoko
AU2 - Oyama, Takuji
AU3 - Mayanagi, Kouta
AU4 - Ishino, Sonoko
AU5 - Ishino, Yoshizumi
AU6 - Morikawa, Kosuke
AB - DNA polymerase requires two processing factors, sliding clamps and clamp loaders, to direct rapid and accurate duplication of genomic DNA. In eukaryotes, proliferating cell nuclear antigen (PCNA), the ring-shaped sliding clamp, encircles double-stranded DNA within its central hole and tethers the DNA polymerases onto DNA. Replication factor C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands in an ATP-dependent manner. Here we report the three-dimensional structure of an archaeal clamp-loading complex (RFC–PCNA–DNA) determined by single-particle EM. The three-dimensional structure of the complex, reconstituted in vitro using a nonhydrolyzable ATP analog, reveals two components, a closed ring and a horseshoe-shaped element, which correspond to PCNA and RFC, respectively. The atomic structure of PCNA fits well into the closed ring, suggesting that this ternary complex represents a state just after the PCNA ring has closed to encircle the DNA duplex.
TI - The clamp-loading complex for processive DNA replication
JF - Nature Structural & Molecular Biology
DO - 10.1038/nsmb788
DA - 2004-06-20
UR - https://www.deepdyve.com/lp/springer-journals/the-clamp-loading-complex-for-processive-dna-replication-evPt4hodig
SP - 632
EP - 636
VL - 11
IS - 7
DP - DeepDyve
ER -