TY - JOUR
AU - Trivedi, Prabodh Kumar
AB - Withania somnifera, a valuable medicinal plant, synthesizes low molecular weight secondary metabolites known as withanolides with beneficial activities towards several diseases. The study of gene expression profiles and the development of EST datasets have developed an understanding of biosynthetic pathways for withanolides to some extent. However, molecular mechanisms regulating this biosynthetic pathway have yet to be studied in detail. In the present study, two transcription factors, WsMYBL1 and WsMYBL2, with contrasting differences in expression in root and leaf tissues, have been used to demonstrate their role in the biosynthesis of withanolides. Using the Virus-Induced Gene Silencing Approach (VIGS), the silencing of both transcription factors modulated plant phenotype and the expression of genes involved in the terpenoid pathway. This led to changes in total and specific withanolide content in the leaf and root tissues of silenced plants compared to the control. In conclusion, our study suggests the role of WsMYBL1 and WsMYBL2 as negative regulators of the withanolides biosynthesis pathway in Withania somnifera.
TI - Involvement of MYB family transcription factors, WsMYBL1 and WsMYBL2, in withanolide biosynthesis in Withania somnifera, a medicinal plant
JF - Plant Cell Tissue and Organ Culture (PCTOC)
DO - 10.1007/s11240-024-02681-0
DA - 2024-02-01
UR - https://www.deepdyve.com/lp/springer-journals/involvement-of-myb-family-transcription-factors-wsmybl1-and-wsmybl2-in-fxD001HJh2
VL - 156
IS - 2
DP - DeepDyve
ER -