TY - JOUR
AU1 - Ellegren,, Hans
AB - Abstract Genomic DNA libraries of the Barn Swallow (Hirundo rustica) and the Pied Flycatcher (Ficedula hypoleuca) were screened for the presence of dinucleotide microsatellite repeats. Two thymine-guanine repeats, “(TG)n,” and two thymine-cytosine repeats, “(TC)n,” were isolated and sequenced from the two species, respectively. Polymerase-chain-reaction (PCR) analysis of 25 unrelated Barn Swallows and 10 unrelated Pied Flycatchers revealed 3 to 15 alleles per locus and heterozygosities in the range of 0.46 to 0.89. Mendelian inheritance was confirmed for all four loci in 10 Pied Flycatcher and 2 Barn Swallow families comprising a total of 240 meioses. The occurrence of nonparental alleles in offspring from two Barn Swallow families was consistent with extrapair fertilization as revealed by a parallel DNA fingerprinting analysis. A DNA amount approximately corresponding to 0.01 μl blood was used for PCR analysis. DNA was also prepared from feathers and approximately 1% of a preparation from a single remex or rectrix was sufficient for PCR amplification. One of the primer pairs from the Barn Swallow also amplified a polymorphic locus in the House Martin (Delichon urbica) and in the Bank Swallow (R. riparia). This content is only available as a PDF. Copyright © 1992 by American Ornithological Society Inc. All rights reserved.
TI - Polymerase-Chain-Reaction (PCR) Analysis of Microsatellites-A New Approach to Studies of Genetic Relationships in Birds
JF - Auk: Ornithological Advances
DO - 10.2307/4088163
DA - 1992-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/polymerase-chain-reaction-pcr-analysis-of-microsatellites-a-new-gbO3En20AL
SP - 886
EP - 895
VL - 109
IS - 4
DP - DeepDyve
ER -