TY - JOUR
AU - Shaw, Andrey S.
AB - Although the Src family tyrosine kinase Lck is essential for T cell receptor (TCR) signaling, whether or how Lck is activated is unknown. Using a phosphospecific antiserum to Lck, we show here that Lck becomes autophosphorylated when T cells are stimulated by antigen-presenting cells (APCs). We found that TCR cross-linking alone could not stimulate Lck autophosphorylation and CD45 was not required for this process. Instead, the T cell accessory molecules CD4 and CD28 cooperated to induce autophosphorylation of Lck. CD4 recruited Lck to the T cell–APC interface, whereas CD28 sustained Lck activation. These data show how the multiple interactions afforded by the immunological synapse drive efficient and highly specific signaling.
TI - Regulation of Lck activity by CD4 and CD28 in the immunological synapse
JF - Nature Immunology
DO - 10.1038/ni761
DA - 2002-02-04
UR - https://www.deepdyve.com/lp/springer-journals/regulation-of-lck-activity-by-cd4-and-cd28-in-the-immunological-i5x7wDDlTY
SP - 259
EP - 264
VL - 3
IS - 3
DP - DeepDyve
ER -