TY - JOUR
AU - Cho, Hyung-Taeg
AB - Understanding the cellular differentiation of multicellular organisms requires the characterization of genes whose expression is modulated in a cell type-specific manner. The Arabidopsis (Arabidopsis thaliana) root hair cell is one model for studying cellular differentiation. In this study, root hair cell-specific genes were screened by a series of in silico and experimental filtration procedures. This process included genome-wide screening for genes with a root hair-specific cis-element in their promoters, filtering root-specific genes from the root hair-specific cis-element-containing genes, further filtering of genes that were suppressed in root hair-defective plant lines, and experimental confirmation by promoter assay. These procedures revealed 19 root hair-specific genes, including many protein kinases and cell wall-related genes, most of which have not been characterized thus far. Functional analyses of these root hair-specific genes with loss-of-function mutants and overexpressing transformants revealed that they play roles in hair growth and morphogenesis. This study demonstrates that a defined cis-element can serve as a filter to screen certain cell type-specific genes and implicates many new root hair-specific genes in root hair development.
TI - cis-Element- and Transcriptome-Based Screening of Root Hair-Specific Genes and Their Functional Characterization in Arabidopsis   
JF - PLANT PHYSIOLOGY
DO - 10.1104/pp.109.140905
DA - 2009-05-15
UR - https://www.deepdyve.com/lp/oxford-university-press/cis-element-and-transcriptome-based-screening-of-root-hair-specific-igzSBw5Kw9
SP - 1459
EP - 1473
VL - 150
IS - 3
DP - DeepDyve
ER -