TY - JOUR
AU1 - Oberstrass, Florian C
AU2 - Auweter, Sigrid D
AU3 - Erat, Michèle
AU4 - Hargous, Yann
AU5 - Henning, Anke
AU6 - Wenter, Philipp
AU7 - Reymond, Luc
AU8 - Amir-Ahmady, Batoul
AU9 - Pitsch, Stefan
AU1 - Black, Douglas L
AU1 - Allain, Frédéric H-T
AB - The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.
TI - Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
JF - Science (New York, N.Y.)
DO - 10.1126/science.1114066
DA - 2005-10-20
UR - https://www.deepdyve.com/lp/pubmed/structure-of-ptb-bound-to-rna-specific-binding-and-implications-for-j7uKB8nAHQ
SP - 2054
EP - 7
VL - 309
IS - 5743
DP - DeepDyve
ER -