TY - JOUR
AU - YOKOMURA,, E-ITI
AB - Abstract Primary leaves of intact bean plants (Phaseolus vulgaris L. cv. Yamashiro-kurosando-saito) were treated with benzyladenine (BA) beginning on the seventh day after sowing when cell proliferation in the leaves had finished. Nuclear DNA contents were measured by cytofluorometry combined with 4',6-diamidino-2-phenylindole (DAPI) staining. In the untreated controls, most mesophyll and abaxial epidermal cells contained a nucleus whose DNA content was 2C; whereas most adaxial epidermal cells contained a 4C nucleus. Benzyladenine treatment induced 4C nuclec in mesophyll and abaxial epidermal cells; but BA induced 8C nuclei in adaxial epidermal cells. To compare the effects of endogenous cytokinin accumulation, bean plants were decapitated above the primary leaves on day 7 and continually disbudded thereafter. Changes in the nuclear DNA content in primary leaves attached to the decapitated plants was similar to that for BA-treated primary leaves. No multinucleate cells were formed and no mitotic figures were present in the BA-treated leaves or in the primary leaves of decapitated plants. Our results indicate that both BA treatment and decapitation induced one round of nuclear DNA synthesis without mitosis in a large number of mesophyll and epidermal cells. This content is only available as a PDF. © Oxford University Press.
TI - Increases in Nuclear DNA Content without Mitosis in Benzyladenine-treated Primary Leaves of Intact and Decapitated Bean Plants
JO - Journal of Experimental Botany
DO - 10.1093/jxb/42.5.667
DA - 1991-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/increases-in-nuclear-dna-content-without-mitosis-in-benzyladenine-kLG08Vd2vL
SP - 667
EP - 672
VL - 42
IS - 5
DP - DeepDyve
ER -