TY - JOUR
AU - Zhou, Qi
AB - TGG TCC C o RRESP o NDENCE small RNA that can be simply expressed or Simultaneous generation and transfected with the Cas9 nuclease. We chose the Tet family genes as targets germline transmission of multiple to demonstrate the feasibility of CRISPR- Cas–mediated mutagenesis in rat. The gene mutations in rat using three Tet genes encode proteins with DNA hydroxymethylase functions and CRISPR-Cas systems play key roles in the regulation of many important biological processes . The Cas9 endonuclease functions either with a To the Editor: The laboratory rat is an important model combination of a short crRNA and a trans- CRISPRs are clustered, regularly interspaced, organism for human disease research. activating crRNA (tracrRNA), or with a short palindromic repeats present in many Several technologies including zinc finger chimeric single guide RNA (sgRNA). We bacteria and archaea genomes. Proteins nuclease (ZFN), transcription activator-like designed six sgRNAs targeting six different encoded by CRISPR-associated (Cas) genes effector nuclease (TALEN) and isolation genomic sites encoding rat Tet1 (sgTet1-1 and serve as guardians of the genome, which of rat embryonic stem cells have enabled sgTet2-1), Tet2 (sgTet2-1 and sgTet2-2) and 8–10 target foreign DNA at specific sites by means targeted gene mutation of the 
TI - Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems
JF - Nature Biotechnology
DO - 10.1038/nbt.2652
DA - 2013-08-08
UR - https://www.deepdyve.com/lp/springer-journals/simultaneous-generation-and-germline-transmission-of-multiple-gene-lqv5G4tpJE
SP - 684
EP - 686
VL - 31
IS - 8
DP - DeepDyve
ER -