TY - JOUR
AU - Lyden, David
AB - npg Exosomes carry double-stranded DNA Cell Research (2014) 24:766-769. npg © 2014 IBCB, SIBS, CAS All rights reserved 1001-0602/14 www.nature.com/cr LETTER TO THE EDITOR Double-stranded DNA in exosomes: a novel biomarker in cancer detection Cell Research (2014) 24:766-769. doi:10.1038/cr.2014.44; published online 8 April 2014 otides and Lambda dsDNA as substrates (Supplementary Dear Editor, information, Figure S1A). Slight non-specificity of S1 Exosomes, small membrane vesicles (30-100 nm) nuclease towards dsDNA was observed. First, to analyze of endocytic origin secreted by most cell types, contain whether DNA is associated with the outer membrane functional biomolecules, which can be horizontally and/or inside exosomes, we extracted DNA from either transferred to recipient cells [1]. Exosomes bear a spe- intact exosomes or exosomes pre-treated with DNases. cific protein and lipid composition, and carry a select Remarkably, we observed no change in the pattern of set of functional mRNAs and microRNAs [2]. Recently, DNA isolated from samples pre-treated with S1 nuclease our group has shown that c-Met shed in exosomes can versus untreated samples (Figure 1A, lane 2 vs lane 5), promote a proangiogenic and prometastatic phenotype in whereas exosomes pre-treated with dsDNase showed a bone marrow-derived progenitor cells during melanoma strong reduction in DNA 
TI - Double-stranded DNA in exosomes: a novel biomarker in cancer detection
JF - Cell Research
DO - 10.1038/cr.2014.44
DA - 2014-04-08
UR - https://www.deepdyve.com/lp/springer-journals/double-stranded-dna-in-exosomes-a-novel-biomarker-in-cancer-detection-nqQLBjOaSV
SP - 766
EP - 769
VL - 24
IS - 6
DP - DeepDyve
ER -