TY - JOUR
AU1 - Kofanova, Olga
AU2 - Henry, Estelle
AU3 - Aguilar Quesada, Rocio
AU4 - Bulla, Alexandre
AU5 - Navarro Linares, Hector
AU6 - Lescuyer, Pierre
AU7 - Shea, Kathi
AU8 - Stone, Mars
AU9 - Tybring, Gunnel
AU1 - Bellora, Camille
AU1 - Betsou, Fay
AB - AbstractBackground:Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified.Methods:Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their “diagnostic performance” in identifying serum or plasma samples with extended pre-centrifugation times.Results:In this study, using R&D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL.Conclusions:These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.
TI - IL8 and IL16 levels indicate serum and plasma quality
JF - Clinical Chemistry and Laboratory Medicine (CCLM)
DO - 10.1515/cclm-2017-1047
DA - 2018-06-27
UR - https://www.deepdyve.com/lp/de-gruyter/il8-and-il16-levels-indicate-serum-and-plasma-quality-pcSv9c9SSn
SP - 1054
EP - 1062
VL - 56
IS - 7
DP - DeepDyve
ER -