TY - JOUR
AU1 - Bauer, Mario
AU2 - Schilling,  Nicole
AU3 - Spanel-Borowski,  Katharina
AB - Microwave treatment (MW) involves completely blocking contaminating staining in the double-labelling technique, using primary monoclonal antibodies from the same species and the same isotype as well as the same secondary antibody (ab). However, we noticed some limitations when locating proliferating cell types in cryostat and paraffin sections using the advantages presented by MW. Control experiments have shown that MW does not diminish contaminating staining when cytoplasmic (desmin, ASM-1) or nuclear (Ki-67) antigens have been labelled with antibodies in the first round of immunolabelling. In contrast to the cell surface antigen, CD18, where the primary ab had to be crosslinked by a secondary ab to obtain contaminating staining, this was observed for the detection of cytoplasmic or nuclear antigens only labelled with a primary ab. In conclusion, for double immunolabelling with abs from the same species and the same isotype, MW is not able to completely abolish contaminating staining.
TI - Limitation of microwave treatment for double immunolabelling with antibodies of the same species and isotype
JF - Histochemistry and Cell Biology
DO - 10.1007/s004180100309
DA - 2001-09-01
UR - https://www.deepdyve.com/lp/springer-journals/limitation-of-microwave-treatment-for-double-immunolabelling-with-qew9N9QqHV
SP - 227
EP - 232
VL - 116
IS - 3
DP - DeepDyve
ER -