TY - JOUR
AU1 - Kalliokoski, Suvi
AU2 - Piqueras, Victoria
AU3 - Frías, Rafael
AU4 - Sulic, Ana-Marija
AU5 - Määttä, Juha
AU6 - Kähkönen, Niklas
AU7 - Viiri, Keijo
AU8 - Huhtala, Heini
AU9 - Pasternack, Arja
AU1 - Laurila, Kaija
AU1 - Sblattero, Daniele
AU1 - Korponay-Szabó, Ilma
AU1 - Mäki, Markku
AU1 - Caja, Sergio
AU1 - Kaukinen, Katri
AU1 - Lindfors, Katri
AB - Coeliac disease is hallmarked by an abnormal immune reaction against ingested wheat-, rye- and barley-derived gluten and the presence of transglutaminase 2 (TG2)-targeted autoantibodies. The small-bowel mucosal damage characteristic of the disorder develops gradually from normal villus morphology to inflammation and finally to villus atrophy with crypt hyperplasia. Patients with early-stage coeliac disease have TG2-autoantibodies present in serum and small-intestinal mucosa and they may already suffer from abdominal symptoms before the development of villus atrophy. Previously, we have shown that intraperitoneal injections of coeliac patient-derived sera or purified immunoglobulin fraction into mice induce a condition mimicking early-stage coeliac disease. In the current study, we sought to establish whether recombinantly produced patient-derived TG2-targeted autoantibodies are by themselves sufficient for the development of such an experimentally induced condition in immune-compromised mice. Interestingly, mice injected with coeliac patient TG2-antibodies had altered small-intestinal mucosal morphology, increased lamina propria cellular infiltration and disease-specific autoantibodies deposited in the small bowel, but did not evince clinical features of the disease. Thus, coeliac patient-derived TG2-specific autoantibodies seem to be sufficient for the induction of subtle small-bowel mucosal alterations in mice, but the development of clinical features probably requires additional factors such as other antibody populations relevant in coeliac disease.
TI - Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice
JF - Amino Acids
DO - 10.1007/s00726-016-2306-0
DA - 2016-08-09
UR - https://www.deepdyve.com/lp/springer-journals/transglutaminase-2-specific-coeliac-disease-autoantibodies-induce-t70EfiFM0G
SP - 529
EP - 540
VL - 49
IS - 3
DP - DeepDyve
ER -