TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on November 4, 2021 - Published by Cold Spring Harbor Laboratory Press E2F integrates cell cycle progression with DNA repair, replication, and G /M checkpoints 1,4,5 3,4 3 1 1 Bing Ren, Hieu Cam, Yasuhiko Takahashi, Thomas Volkert, Jolyon Terragni, 1,2 3,6 Richard A. Young, and Brian David Dynlacht 1 2 Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; Massachusetts Institute of Technology, Department of Biology, Cambridge, Massachusetts 02139, USA; Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA The E2F transcription factor family is known to play a key role in the timely expression of genes required for cell cycle progression and proliferation, but only a few E2F target genes have been identified. We explored the possibility that E2F regulators play a broader role by identifying additional genes bound by E2F in living human cells. A protocol was developed to identify genomic binding sites for DNA-binding factors in mammalian cells that combines immunoprecipitation of cross-linked protein–DNA complexes with DNA microarray analysis. Among ∼1200 genes expressed during cell cycle entry, we found that the promoters of 127 were bound by the E2F4 transcription factor in primary fibroblasts. A subset of these targets 
TI - E2F integrates cell cycle progression with DNA repair, replication, and G2/M checkpoints
JF - Genes & Development
DO - 10.1101/gad.949802
DA - 2002-01-15
UR - https://www.deepdyve.com/lp/unpaywall/e2f-integrates-cell-cycle-progression-with-dna-repair-replication-and-tBrlZaaqIY
DP - DeepDyve
ER -