TY - JOUR AU - AB - Downloaded from genesdev.cshlp.org on November 2, 2021 - Published by Cold Spring Harbor Laboratory Press atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes 1 1 1 2 2 Sergiy Lopato, Maria Kalyna, Silke Dorner, Ryuji Kobayashi, Adrian R. Krainer, 1,3 and Andrea Barta 1 2 Institut fu ¨ r Biochemie, Universita ¨ t Wien, Vienna Biocenter, A-1030 Vienna, Austria; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724-2208 USA SR proteins are nuclear phosphoproteins with a characteristic Ser/Arg-rich domain and one or two RNA recognition motifs. They are highly conserved in animals and plants and play important roles in spliceosome assembly and alternative splicing regulation. We have now isolated and partially sequenced a plant protein, which crossreacts with antibodies to human SR proteins. The sequence of the corresponding cDNA and genomic clones from Arabidopsis revealed a protein, atSRp30, with strong similarity to the human SR protein SF2/ASF and to atSRp34/SR1, a previously identified SR protein, indicating that plants possess two SF2/ASF-like proteins. atSRp30 expresses alternatively spliced mRNA isoforms that are expressed differentially in various organs and during development. Overexpression of atSRp30 via a strong constitutive promoter resulted in changes in alternative TI - atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes JF - Genes & Development DO - 10.1101/gad.13.8.987 DA - 1999-04-15 UR - https://www.deepdyve.com/lp/unpaywall/atsrp30-one-of-two-sf2-asf-like-proteins-from-arabidopsis-thaliana-tChgSdDf70 DP - DeepDyve ER -