TY - JOUR
AU1 - Yi, Xiaochun
AU2 - Zhang, Jie
AU3 - Liu, Huixiang
AU4 - Yi, Tianxia
AU5 - Ou, Yuhua
AU6 - Liu, Meilan
AU7 - Zhu, Liqiong
AU8 - Chen, Hui
AU9 - Zhang, Jianping
AB - The adverse clinical result and poor treatment outcome in recurrent spontaneous abortion (RSA) make it necessary to understand the pathogenic mechanism. The mating combination CBA/J × DBA/2 has been widely used as an abortion-prone model compared to DBA/2-mated CBA/J mice. Here, we used RNA-seq to get a comprehensive catalogue of genes differentially expressed between survival placenta in abortion-prone model and control. Five hundred twenty-four differentially expressed genes were obtained followed by clustering analysis, Gene Ontology analysis, and pathway analysis. We paid more attention to immune-related genes namely “immune response” and “immune system process” including 33 downregulated genes and 28 upregulated genes. Twenty-one genes contribute to suppressing immune system and 7 are against it. Six genes were validated by reversetranscription-polymerasechain reaction, namely Ccrlll, Tlr4, Tgf-β1,Tyro3, Gzmb, and Il-1β. Furthermore, Tlr4, Tgf-β1, and Il-Iβ were analyzed by Western blot. Such immune profile gives us a better understanding of the complicated immune processing in RSA and immunosuppression can rescue pregnancy loss.
TI - Suppressed Immune-Related Profile Rescues Abortion-Prone Fetuses: A Novel Insight Into the CBA/J × DBA/2J Mouse Model
JF - Reproductive Sciences
DO - 10.1177/1933719119828042
DA - 2021-02-25
UR - https://www.deepdyve.com/lp/springer-journals/suppressed-immune-related-profile-rescues-abortion-prone-fetuses-a-trDEKduDVV
SP - 1485
EP - 1492
VL - 26
IS - 11
DP - DeepDyve
ER -