TY - JOUR
AU1 - Kirchhof, B.
AU2 - Schmale, S.
AU3 - van de Loo, J.
AB - In contrast to earlier assays, the prothrombin determination described here uses a two-step reaction: first, prothrombin is activated with human factor Xa, phospholipids, Ca<sup>++</sup> and factor V; second, the amount of activated prothrombin is assayed by the proteolysis of added fibrinogen. The assay is dependent only on prothrombin, and independent of other components of thrombin generation. Carboxy- and decarboxyprothrombin can be differentiated by means of using Echis carinatus venom instead of factor Xa as activator. The micro-coagulation assay is compared with a conventional one-stage coagulation test, with a prothrombin determination using chromogenic substrate and immunologically by Laurell electrophoresis.
TI - Determination of Prothrombin by a Micro-Coagulation Method
JF - Pathophysiology of Haemostasis and Thrombosis
DO - 10.1159/000214697
DA - 1983-01-01
UR - https://www.deepdyve.com/lp/karger/determination-of-prothrombin-by-a-micro-coagulation-method-vPwbTppErT
SP - 1
EP - 8
VL - 13
IS - 1
DP - DeepDyve
ER -