TY - JOUR
AU - 
AB - Downloaded from genesdev.cshlp.org on December 1, 2021 - Published by Cold Spring Harbor Laboratory Press The 65-kD subunit of NF-KB is a receptor for IKB and a modulator of DNA-binding specificity Manuela B. Urban and Patrick A. Baeuerle^ Laboratorium fur Molekulare Biologic der Ludwig-Maximilians-Universitat Miinchen, Genzentrum D-8033 Martinsried, Germany A recent study has shown that the dimeric form of the 50-kD DNA-binding subunit of the NF-KB transcription factor is commonly associated with two molecules of a 65-kD protein (p65) and that p65 appears to be required for inactivation of the p50 dimer by the inhibitory subunit IKB. Here^ we provide evidence that p65 serves as a receptor for IKB. Preincubation of IKB with purified p65 prevented IKB from inactivating the heterotetrameric form of NF-KB. Furthermore, excess p65 could very efficiently activate the latent form of NF-KB composed of p50, p65, and IKB, presumably by binding the IKB, which was released from the complex due to its inherent off rate. An additional function of p65 in modulating the DNA-binding specificity of p50 was found. In the absence of p65, p50 could bind with high affinity to completely palindromic sites. The heterotetramer recognized these sites with an affinity > 10-fold lower 
TI - The 65-kD subunit of NF-kappa B is a receptor for I kappa B and a modulator of DNA-binding specificity.
JF - Genes & Development
DO - 10.1101/gad.4.11.1975
DA - 1990-11-01
UR - https://www.deepdyve.com/lp/unpaywall/the-65-kd-subunit-of-nf-kappa-b-is-a-receptor-for-i-kappa-b-and-a-w2dTzn0Yto
DP - DeepDyve
ER -