TY - JOUR
AU1 - Yu, Xiaona
AU2 - Kang, Dong
AU3 - Choi, Su
AU4 - Ma, Yinbo
AU5 - Lu, Lu
AU6 - Oh, Sang
AU7 - Chhapekar, Sushil
AU8 - Lim, Yong
AB - The resistance gene analog (RGA)-based marker strategy is an effective supplement for current marker reservoir of radish disease-resistance breeding. In this study, we identified RGAs based on the conserved nucleotide-binding site (NBS) and S-receptor-like kinase (SRLK) domains. A total of 68 NBS-RGAs and 46 SRLK-RGAs were isolated from two FW-resistant radish inbred lines, B2 and YR31, and one susceptible line, YR15. A BLASTx search revealed that the NBS-RGAs contained six conserved motifs (i.e., P loop, RNBS-A, Kinase-2, RNBS-B, RNBS-C, and GLPL) and the SRLK-RGAs, contained two conserved motifs (i.e., G-type lectin and PAN-AP). A phylogenetic analysis indicated that the NBS-RGAs could be separated into two classes (i.e., toll/interleukin receptor and coiled-coil types), with six subgroups, and the SRLK-RGAs were divided into three subgroups. Moreover, we designed RGA-specific markers from data-mining approach in radish databases. Based on marker analysis, 24 radish inbred lines were clustered into five main groups with a similarity index of 0.44 and showing genetic diversity with resistance variation in those radish inbred lines. The development of RGA-specific primers would be valuable for marker-assisted selection during the breeding of disease-resistant radish cultivars.
TI - Isolation and characterization of fusarium wilt resistance gene analogs in radish
JF - 3 Biotech
DO - 10.1007/s13205-018-1279-y
DA - 2018-05-14
UR - https://www.deepdyve.com/lp/springer-journals/isolation-and-characterization-of-fusarium-wilt-resistance-gene-yem8zJm4YS
SP - 1
EP - 9
VL - 8
IS - 5
DP - DeepDyve
ER -