Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/detection-of-differentially-expressed-genes-in-prostate-cancer-by-09k5wEel65
References (34)
-
L. Augenlicht, John Taylor, Leigh Anderson, Martin Lipkin (1991)
Patterns of gene expression that characterize the colonic mucosa in patients at genetic risk for colonic cancer.Proceedings of the National Academy of Sciences of the United States of America, 88
-
Jeremy Chen, Jeremy Chen, Reen Wu, P. Yang, P. Yang, Jane Huang, Y. Sher, Meng Han, W. Kao, Pei Lee, Trai-Fu Chiu, Fu Chang, Yi Chu, Cheng-Wen Wu, Cheng-Wen Wu, Konan Peck (1998)
Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection.Genomics, 51 3
-
M. Schena, D. Shalon, R. Heller, A. Chai, P. Brown, R. Davis (1996)
Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.Proceedings of the National Academy of Sciences of the United States of America, 93 20
-
M. Kai, I. Wada, S. Imai, F. Sakane, H. Kanoh (1997)
Cloning and Characterization of Two Human Isozymes of Mg2+-independent Phosphatidic Acid Phosphatase*The Journal of Biological Chemistry, 272
-
F. Rastinejad, M. Conboy, T. Rando, H. Blau (1993)
Tumor suppression by RNA from the 3′ untranslated region of α-tropomyosinCell, 75
-
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual -
Ji Huang, Hongsheng Zhang, Dong Wang, Ya-Jun Cao, Jin-shui Yang (2002)
[Serial analysis of gene expression].Yi chuan = Hereditas, 24 2
-
D. Lockhart, Helin Dong, M. Byrne, M. Follettie, Michael Gallo, M. Chee, Mike Mittmann, Chunwei Wang, M. Kobayashi, Heidi Norton, E. Brown (1996)
Expression monitoring by hybridization to high-density oligonucleotide arraysNature Biotechnology, 14
-
M. Hubank, D. Schatz (1994)
Identifying differences in mRNA expression by representational difference analysis of cDNA.Nucleic acids research, 22 25
-
Y. Oh, S. Nagalla, Y. Yamanaka, Ho-Seong Kim, E. Wilson, R. Rosenfeld (1996)
Synthesis and Characterization of Insulin-like Growth Factor-binding Protein (IGFBP)-7The Journal of Biological Chemistry, 271
-
Y Oh, SR Nagalla, Y Yamanaka, HS Kim, E Wilson, RG Rosenfeld (1996)
Synthesis and characterization of insulin‐like growth factor‐binding protein (IGFBP)‐7. Recombinant human mac25 protein specifically binds IGF‐I and ‐II, 271
-
S. Hedrick, D. Cohen, E. Nielsen, Mark Davis (1984)
Isolation of cDNA clones encoding T cell-specific membrane-associated proteinsNature, 308
-
M. Schena, D. Shalon, Ronald Davis, P. Brown (1995)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayScience, 270
-
AP Stubbs, PD Abel, M Golding (1999)
Differentially expressed genes in hormone refractory prostate cancer, 154
-
J. Zhang, M. Nelson, Liang Wang, Wanguo Liu, C. Qian, V. Shridhar, R. Urrutia, David Smith (1998)
Identification and Chromosomal Localization of CTNNAL1, a Novel Protein Homologous to α-CateninGenomics, 54
-
L. Diatchenko, Yun-Fai Lau, Aaron Campbell, A. Chenchik, Fauzia Moqadam, B. Huang, S. Lukyanov, K. Lukyanov, N. Gurskaya, E. Sverdlov, P. Siebert (1996)
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.Proceedings of the National Academy of Sciences of the United States of America, 93 12
-
P. Nelson, L. Gan, C. Ferguson, P. Moss, R. Gelinas, Leroy Hood, Kai Wang (1999)
Molecular cloning and characterization of prostase, an androgen-regulated serine protease with prostate-restricted expression.Proceedings of the National Academy of Sciences of the United States of America, 96 6
-
N. Lisitsyn, Natalya Lisitsyn, M. Wigler (1993)
Cloning the differences between two complex genomesScience, 259
-
D. Leung, C. Tompkins, T. White (1998)
Molecular cloning of two alternatively spliced forms of human phosphatidic acid phosphatase cDNAs that are differentially expressed in normal and tumor cells.DNA and cell biology, 17 4
-
A. Stubbs, P. Abel, M. Golding, G. Bhangal, Qin Wang, J. Waxman, G. Stamp, E. Lalani (1999)
Differentially expressed genes in hormone refractory prostate cancer: association with chromosomal regions involved with genetic aberrations.The American journal of pathology, 154 5
-
George Yang, D. Ross, Wayne Kuang, P. Brown, R. Weigel (1999)
Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes.Nucleic acids research, 27 6
-
H. Jin, X. Cheng, L. Diatchenko, P. Siebert, C. Huang (1997)
Differential screening of a subtracted cDNA library: a method to search for genes preferentially expressed in multiple tissues.BioTechniques, 23 6
-
N. Nupponen, E. Hyytinen, A. Kallioniemi, T. Visakorpi (1998)
Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization.Cancer genetics and cytogenetics, 101 1
-
V. Iyer, M. Eisen, D. Ross, Greg Schuler, T. Moore, Jeffrey Lee, J. Trent, L. Staudt, J. Hudson, M. Boguski, D. Lashkari, D. Shalon, D. Botstein, P. Brown (1999)
The transcriptional program in the response of human fibroblasts to serum.Science, 283 5398
-
Peter Nelson, S. Plymate, Kai Wang, Lawrence True, Joy Ware, Lu Gan, Alvin Liu, Leroy Hood (1998)
Hevin, an antiadhesive extracellular matrix protein, is down-regulated in metastatic prostate adenocarcinoma.Cancer research, 58 2
-
A. Degeorges, Fuan Wang, H. Frierson, A. Seth, Leland Chung, R. Sikes (1999)
Human prostate cancer expresses the low affinity insulin-like growth factor binding protein IGFBP-rP1.Cancer research, 59 12
-
J. Derisi, L. Penland, P. Brown, M. Bittner, P. Meltzer, M. Ray, Yidong Chen, Y. Su, J. Trent (1996)
Use of a cDNA microarray to analyse gene expression patterns in human cancerNature Genetics, 14
-
M. Yang, A. Sytkowski (1996)
Cloning differentially expressed genes by linker capture subtraction.Analytical biochemistry, 237 1
-
Peng Liang, A. Pardee (1992)
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.Science, 257 5072
-
N. Hastie, J. Bishop (1976)
The expression of three abundance classes of messenger RNA in mouse tissuesCell, 9
-
N. Nupponen, Kati Porkka, L. Kakkola, M. Tanner, Karin Persson, Å. Borg, J. Isola, T. Visakorpi (1999)
Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer.The American journal of pathology, 154 6
-
D. Duggan, M. Bittner, Yidong Chen, P. Meltzer, J. Trent (1999)
Expression profiling using cDNA microarraysNature Genetics, 21 Suppl 1
-
Leena Pulkkinen, T. Alitalo, T. Krusius, L. Peltonen (1992)
Expression of decorin in human tissues and cell lines and defined chromosomal assignment of the gene locus (DCN).Cytogenetics and cell genetics, 60 2
-
Fanan Rastinejad, M. Conboy, T. Rando, H. Blau (1993)
Tumor suppression by RNA from the 3' untranslated region of alpha-tropomyosin.Cell, 75 6
- Publisher
- Wiley
- Copyright
- Copyright © 2001 Wiley Subscription Services
- ISSN
- 0022-3417
- eISSN
- 1096-9896
- DOI
- 10.1002/1096-9896(2000)9999:9999<::AID-PATH751>3.0.CO;2-Y
- pmid
- 11169518
- Publisher site
- See Article on Publisher Site
Abstract
The molecular mechanisms underlying the development and progression of prostate cancer have remained poorly understood. The identification of differentially expressed genes has been used as a tool to recognize genes that are involved in disease processes. In this study we combined suppression subtractive hybridization (SSH) and cDNA array hybridization to identify genes whose expression is decreased in prostate cancer. cDNA from benign prostatic hyperplasia (BPH) was subtracted with cDNA from the prostate cancer cell line PC‐3 and 386 of the subtracted clones were arrayed onto a nylon filter membrane. The differential gene expression was then verified by hybridizing the filter with radioactively labelled first‐strand cDNA preparations from BPH, PC‐3, four other cancer cell lines, and a normal prostate epithelial cell line (PrEC). In order to validate SSH and cDNA array hybridization, the enrichment of clones in the subtraction, as well as the sensitivity and linearity of array hybridization, was first evaluated. The array hydridization results were confirmed by northern analysis and selected clones were sequenced. Altogether, several known genes, such as prostate‐specific antigen (PSA), human glandular kallikrein 2 (hK2), phosphatidic acid phosphatase type 2a (PAP2a), α‐tropomyosin, and insulin‐like growth factor binding protein 7 (IGFBP‐7), as well as an anonymous transcript (EST), were found to be expressed less in PC‐3 than in BPH. Further studies on the significance of these genes in the development of prostate cancer are now warranted. Copyright © 2000 John Wiley & Sons, Ltd.
Journal
The Journal of Pathology – Wiley
Published: Jan 1, 2001
Keywords: ; ; ; ;