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25-Hydroxyvitamin D 3

25-Hydroxyvitamin D 3 Vascular Medicine 25-Hydroxyvitamin D -1-Hydroxylase Is Expressed in Human Vascular Smooth Muscle Cells and Is Upregulated by Parathyroid Hormone and Estrogenic Compounds Dalia Somjen, PhD; Yosef Weisman, MD; Fortune Kohen, PhD; Batya Gayer, MSc; Rona Limor, PhD; Orly Sharon, BSc; Niva Jaccard, MSc; Esther Knoll; Naftali Stern, MD Background—1,25(OH) vitamin D exerts multiple effects in human vascular smooth muscle cells (VSMCs). We 2 3 therefore tested the possibility that VSMCs possess an endogenous 25-hydroxyvitamin D -1-hydroxylase system, the final enzyme in the biosynthetic pathway of 1,25(OH) D . 2 3 Methods and Results—We assessed the expression and activity of 25-hydroxyvitamin D -1-hydroxylase by real-time polymerase chain reaction and the conversion of 25(OH)D into 1,25(OH) D . First, 25-hydroxyvitamin D -1- 3 2 3 3 hydroxylase mRNA was identified in cultured VSMCs by real-time polymerase chain reaction. Second, in cells treated daily (3 days) with parathyroid hormone (66 nmol/L), estradiol-17 (30 nmol/L), raloxifene (3 mol/L), and the phytoestrogens genistein (3 mol/L), biochainin A (3 mol/L), and 6-carboxy biochainin A (30 nmol/L), 25- hydroxyvitamin D -1-hydroxylase mRNA increased by 4313%, (P0.05) 724% (PNS), 17628% (P0.01), 6511% (P0.05), 15224% (P0.01), and 719% (P0.05), respectively. Third, production of 1,25(OH) D from 2 3 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Circulation Wolters Kluwer Health

25-Hydroxyvitamin D 3

Stern, Naftali
Circulation , Volume 111 (13) – Apr 1, 2005
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ISSN
0009-7322
eISSN
1524-4539
DOI
10.1161/01.CIR.0000160353.27927.70
pmid
15795327
Publisher site
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Abstract

Vascular Medicine 25-Hydroxyvitamin D -1-Hydroxylase Is Expressed in Human Vascular Smooth Muscle Cells and Is Upregulated by Parathyroid Hormone and Estrogenic Compounds Dalia Somjen, PhD; Yosef Weisman, MD; Fortune Kohen, PhD; Batya Gayer, MSc; Rona Limor, PhD; Orly Sharon, BSc; Niva Jaccard, MSc; Esther Knoll; Naftali Stern, MD Background—1,25(OH) vitamin D exerts multiple effects in human vascular smooth muscle cells (VSMCs). We 2 3 therefore tested the possibility that VSMCs possess an endogenous 25-hydroxyvitamin D -1-hydroxylase system, the final enzyme in the biosynthetic pathway of 1,25(OH) D . 2 3 Methods and Results—We assessed the expression and activity of 25-hydroxyvitamin D -1-hydroxylase by real-time polymerase chain reaction and the conversion of 25(OH)D into 1,25(OH) D . First, 25-hydroxyvitamin D -1- 3 2 3 3 hydroxylase mRNA was identified in cultured VSMCs by real-time polymerase chain reaction. Second, in cells treated daily (3 days) with parathyroid hormone (66 nmol/L), estradiol-17 (30 nmol/L), raloxifene (3 mol/L), and the phytoestrogens genistein (3 mol/L), biochainin A (3 mol/L), and 6-carboxy biochainin A (30 nmol/L), 25- hydroxyvitamin D -1-hydroxylase mRNA increased by 4313%, (P0.05) 724% (PNS), 17628% (P0.01), 6511% (P0.05), 15224% (P0.01), and 719% (P0.05), respectively. Third, production of 1,25(OH) D from 2 3

Journal

Circulation – Wolters Kluwer Health

Published: Apr 1, 2005

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