Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Both CP15 and CP25 are left as trails behind gliding sporozoites of Cryptosporidium parvum (Apicomplexa)

Both CP15 and CP25 are left as trails behind gliding sporozoites of Cryptosporidium parvum... Abstract Sporozoites of Cryptosporidium parvum were examined after gliding upon glass microscope slides using monoclonal antibodies to the 15 and 25 kDa surface molecules and immunogold-silver enhancement. Both antibodies bound to surface antigen deposited as trails behind parasites, suggesting that both surface molecules are involved in substrate attachment. Crytosporidium parvum, Apicomplexa, Coccidia, Silver enhancement, Sporozoite motility References 1 Stewart M.J. Vanderberg J.P. ( 1988 ) Malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility J. Protozool. , 35 , 389 – 393 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Entzeroth R. Zgrzebski G. Dubremetz J.-F. ( 1989 ) Secretion of trails during gliding motility of Eimeria nieschulzi (Apicomplexa, Coccidia) sporozoites visualized by a monoclonal antibody and immuno-gold-silver enhancement Parasitol. Res. , 76 , 174 – 175 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Arrowood M.J. Sterling C.R. Healy M.C. ( 1991 ) Immunofluorescent microscopical visualization of trails left by gliding Cryptosporidium parvum sporozoites J. Parasitol. , 77 , 315 – 317 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Tilley M. Upton S.J. Fayer R. Barta J.R. Chrissp C.E. Freed P.S. Blagburn B.L. Anderson B.C. Barnard S.M. ( 1991 ) Identification of a 15-kilodalton surface glycoprotein on sporozoites of Cryptosporidium parvum Infect. Immun. , 59 , 1002 – 1007 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Tilley M. Eggleston M.T. Upton S.J. ( 1993 ) Multiple oral inoculations with Cryptosporidium parvum as a means as a means of immunization for production of monoclonal antibodies FEMS Microbiol. Lett. , 113 , 235 – 240 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Taghi-Kilani R. Sekla L. ( 1987 ) Purification of Cryptosporidium parvumoocysts and sporozoites by cesium chloride and percoll gradients Am. J. Trop. Med. Hyg. , 36 , 505 – 508 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Upton S.J. Tilley M. Nesterenko M.V. Brillhart D.B. ( 1994 ) A simple and reliable method of producing in vitro infections of Cryptosporidium parvum (Apicomplexa) FEMS Microbiol. Lett. , 118 , 45 – 50 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Mead J.R. Arrowood M.J. Sterling C.R. ( 1988 ) Antigens of Cryptosporidium sporozoites recognized by immune sera of infected animals and humans J. Parasitol. , 74 , 135 – 143 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Ungar B.L.P. Nash T.E. ( 1986 ) Quantification of specific antibody response by Cryptosporidium antigens by laser densitometry Infect. Immun. , 53 , 124 – 128 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Moss D.M. Lammie P.J. ( 1993 ) Proliferative responsiveness of lymphocytes from Cryptosporidium parvum-exposed mice to two separate antigen fractions from oocysts Am. J. Trop. Med. Hyg. , 49 , 393 – 401 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Whitmire W.M. Harp J.A. ( 1991 ) Characterization of bovine cellular and serum antibody and serum antibody responses during infection by Cryptosporidium parvum Infect. Immun. , 59 , 990 – 995 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 12 Reperant J.-M. Naciri M. Chardes T. Bout D.T. ( 1992 ) Immunological characterization of a 17-kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies FEMS Microbiol. Lett. , 99 , 7 – 14 . Google Scholar Crossref Search ADS WorldCat 13 El-Shewy K. Kilani R.T. Hegazi M.M. Makhlouf L. Wenman W.M. ( 1994 ) Identification of low-molecular-mass coproantigens of Cryptosporidium parvum J. Inf. Dis. , 169 , 460 – 463 . Google Scholar Crossref Search ADS WorldCat This content is only available as a PDF. Author notes 1 Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. © 1994 Federation of European Microbiological Societies http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png FEMS Microbiology Letters Oxford University Press

Both CP15 and CP25 are left as trails behind gliding sporozoites of Cryptosporidium parvum (Apicomplexa)

Tilley,, Michael; Upton, Steve, J.
FEMS Microbiology Letters , Volume 120 (3) – Jul 1, 1994
Download PDF
4 pages

Loading next page...
 
/lp/oxford-university-press/both-cp15-and-cp25-are-left-as-trails-behind-gliding-sporozoites-of-0KaHc0W6Sn

References (14)

Publisher
Oxford University Press
Copyright
© 1994 Federation of European Microbiological Societies
ISSN
0378-1097
eISSN
1574-6968
DOI
10.1111/j.1574-6968.1994.tb07045.x
Publisher site
See Article on Publisher Site

Abstract

Abstract Sporozoites of Cryptosporidium parvum were examined after gliding upon glass microscope slides using monoclonal antibodies to the 15 and 25 kDa surface molecules and immunogold-silver enhancement. Both antibodies bound to surface antigen deposited as trails behind parasites, suggesting that both surface molecules are involved in substrate attachment. Crytosporidium parvum, Apicomplexa, Coccidia, Silver enhancement, Sporozoite motility References 1 Stewart M.J. Vanderberg J.P. ( 1988 ) Malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility J. Protozool. , 35 , 389 – 393 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Entzeroth R. Zgrzebski G. Dubremetz J.-F. ( 1989 ) Secretion of trails during gliding motility of Eimeria nieschulzi (Apicomplexa, Coccidia) sporozoites visualized by a monoclonal antibody and immuno-gold-silver enhancement Parasitol. Res. , 76 , 174 – 175 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Arrowood M.J. Sterling C.R. Healy M.C. ( 1991 ) Immunofluorescent microscopical visualization of trails left by gliding Cryptosporidium parvum sporozoites J. Parasitol. , 77 , 315 – 317 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Tilley M. Upton S.J. Fayer R. Barta J.R. Chrissp C.E. Freed P.S. Blagburn B.L. Anderson B.C. Barnard S.M. ( 1991 ) Identification of a 15-kilodalton surface glycoprotein on sporozoites of Cryptosporidium parvum Infect. Immun. , 59 , 1002 – 1007 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Tilley M. Eggleston M.T. Upton S.J. ( 1993 ) Multiple oral inoculations with Cryptosporidium parvum as a means as a means of immunization for production of monoclonal antibodies FEMS Microbiol. Lett. , 113 , 235 – 240 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Taghi-Kilani R. Sekla L. ( 1987 ) Purification of Cryptosporidium parvumoocysts and sporozoites by cesium chloride and percoll gradients Am. J. Trop. Med. Hyg. , 36 , 505 – 508 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Upton S.J. Tilley M. Nesterenko M.V. Brillhart D.B. ( 1994 ) A simple and reliable method of producing in vitro infections of Cryptosporidium parvum (Apicomplexa) FEMS Microbiol. Lett. , 118 , 45 – 50 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Mead J.R. Arrowood M.J. Sterling C.R. ( 1988 ) Antigens of Cryptosporidium sporozoites recognized by immune sera of infected animals and humans J. Parasitol. , 74 , 135 – 143 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Ungar B.L.P. Nash T.E. ( 1986 ) Quantification of specific antibody response by Cryptosporidium antigens by laser densitometry Infect. Immun. , 53 , 124 – 128 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 10 Moss D.M. Lammie P.J. ( 1993 ) Proliferative responsiveness of lymphocytes from Cryptosporidium parvum-exposed mice to two separate antigen fractions from oocysts Am. J. Trop. Med. Hyg. , 49 , 393 – 401 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Whitmire W.M. Harp J.A. ( 1991 ) Characterization of bovine cellular and serum antibody and serum antibody responses during infection by Cryptosporidium parvum Infect. Immun. , 59 , 990 – 995 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 12 Reperant J.-M. Naciri M. Chardes T. Bout D.T. ( 1992 ) Immunological characterization of a 17-kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies FEMS Microbiol. Lett. , 99 , 7 – 14 . Google Scholar Crossref Search ADS WorldCat 13 El-Shewy K. Kilani R.T. Hegazi M.M. Makhlouf L. Wenman W.M. ( 1994 ) Identification of low-molecular-mass coproantigens of Cryptosporidium parvum J. Inf. Dis. , 169 , 460 – 463 . Google Scholar Crossref Search ADS WorldCat This content is only available as a PDF. Author notes 1 Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. © 1994 Federation of European Microbiological Societies

Journal

FEMS Microbiology Letters – Oxford University Press

Published: Jul 1, 1994

There are no references for this article.