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Molecular Cloning: A Laboratory Manual, 2nd Edn
- Publisher
- Oxford University Press
- Copyright
- © 1998 Federation of European Microbiological Societies.
- ISSN
- 0378-1097
- eISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.1998.tb13046.x
- pmid
- 9673023
- Publisher site
- See Article on Publisher Site
Abstract
AbstractNaturally occurring promoters that are repressed by the Escherichia coli FNR protein are complex. In this work, we have constructed a simple semi-synthetic promoter that is repressed by FNR binding to a single site that overlaps the promoter −35 element. Our results show that a single site for FNR is sufficient for effective repression. This semi-synthetic promoter provides a simple tool for monitoring FNR binding to target sites in the absence of its activation function. We have exploited this to study FNR mutants that are defective in repressing the ndh promoter, a complex naturally occurring promoter that is repressed by FNR.
Journal
FEMS Microbiology Letters – Oxford University Press
Published: Jun 17, 1998