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Extinction Coefficients of Chlorophyll a and b in N,N-Dimethylformamide and 80% Acetone

Extinction Coefficients of Chlorophyll a and b in N,N-Dimethylformamide and 80% Acetone Abstract We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (Chl a) and chlorophyll b (Chl b) in DMF for wavelengths of 618 to 665 nanometers. The simultaneous equations necessary for quantifying Chl a, Chl b, or total Chl in DMF in the absence of other chlorophyllous pigments are: Chl a = 12.70A 664.5 - 2.79A 647; Chl b = 20.70 A 647 - 4.62A 664.5; total Chl = 17.90A 647 + 8.08A 664.5, where A = absorbance in 1.00 centimeter cuvettes and Chl = milligrams per liter. N,N-Dimethylformamide is a very convenient solvent for Chl extraction since it is effective on intact plant parts and Chl is quite stable in DMF. There was no difference in the amount of Chl extracted when plant tissue was stored for 1 or 3 days at three temperatures, with or without solvent added. 1 Scientific Journal Series, paper no. 14,107, Minnesota Agricultural Experiment Station. This content is only available as a PDF. © 1985 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology Oxford University Press

Extinction Coefficients of Chlorophyll a and b in N,N-Dimethylformamide and 80% Acetone

Inskeep, William P.; Bloom, Paul R.
Plant Physiology , Volume 77 (2) – Feb 1, 1985
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References (12)

Publisher
Oxford University Press
Copyright
Copyright © 2021 American Society of Plant Biologists
ISSN
0032-0889
eISSN
1532-2548
DOI
10.1104/pp.77.2.483
Publisher site
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Abstract

Abstract We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (Chl a) and chlorophyll b (Chl b) in DMF for wavelengths of 618 to 665 nanometers. The simultaneous equations necessary for quantifying Chl a, Chl b, or total Chl in DMF in the absence of other chlorophyllous pigments are: Chl a = 12.70A 664.5 - 2.79A 647; Chl b = 20.70 A 647 - 4.62A 664.5; total Chl = 17.90A 647 + 8.08A 664.5, where A = absorbance in 1.00 centimeter cuvettes and Chl = milligrams per liter. N,N-Dimethylformamide is a very convenient solvent for Chl extraction since it is effective on intact plant parts and Chl is quite stable in DMF. There was no difference in the amount of Chl extracted when plant tissue was stored for 1 or 3 days at three temperatures, with or without solvent added. 1 Scientific Journal Series, paper no. 14,107, Minnesota Agricultural Experiment Station. This content is only available as a PDF. © 1985 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Plant Physiology – Oxford University Press

Published: Feb 1, 1985

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