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/lp/oxford-university-press/a-high-performance-liquid-chromatography-radio-organic-acid-analyzer-3IY2jOKsEF
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- Publisher
- Oxford University Press
- Copyright
- Copyright © 1982. The Japanese Society of Plant Physiologists
- ISSN
- 0032-0781
- eISSN
- 1471-9053
- DOI
- 10.1093/oxfordjournals.pcp.a076399
- Publisher site
- See Article on Publisher Site
Abstract
Abstract To monitor rapidly the specific activity of radioactive organic acids, a high performance liquid chromatography (HPLC)-radioanalyzer was constructed. The instrument is a linear combination of an HPLC organic acid analyzer and a radioanalyzer. Organic acids are separated through a 100 cm column packed with Diaion-CK08S (H$type) resin. The organic acids were detected at 430 nm after reaction with bromocresol purple. The effluents from the HPLC are conduced to a U-type flow-cell packed with lithium-glass scintillator, which floresces in response to rrβ-rays. The instrument was utilized to investigate the assimilation of H14CO3− fixation in barley roots after 10 min uptake. Malate showed the highest specific activity, followed by citrate and aspartate. This content is only available as a PDF. Copyright © 1982. The Japanese Society of Plant Physiologists
Journal
Plant and Cell Physiology – Oxford University Press
Published: Jun 1, 1982