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Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core component that functions in chromosome segregation

Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core... Downloaded from genesdev.cshlp.org on November 6, 2021 - Published by Cold Spring Harbor Laboratory Press Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core component that functions in chromosome segregation 1,3 2 2 1 Monique C. Zetka, Ichiro Kawasaki, Susan Strome, and Fritz Mu ¨ ller 1 2 Institute of Zoology, University of Fribourg, Pe ´ rolles, CH-1700 Fribourg, Switzerland; Department of Biology, Indiana University, Bloomington, Indiana 47405 USA Meiotic chromosomes are organized about a proteinaceous core that forms between replicated sister chromatids. We have isolated a Caenorhabditis elegans gene, him-3, which encodes a meiosis-specific component of chromosome cores with some similarity to the yeast lateral element protein Hop1p. Antibodies raised against HIM-3 localize the protein to condensing chromosomes in early prophase I and to the cores of both synapsed and desynapsed chromosomes. In RNA interference experiments, chromosomes appear to condense normally in the absence of detectable protein but fail to synapse and form chiasmata, indicating that HIM-3 is essential for these processes. Hypomorphs of him-3, although being synapsis proficient, show severe reductions in the frequency of crossing-over, demonstrating that HIM-3 has a role in establishing normal levels of interhomolog exchange. Him-3 mutants also show http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core component that functions in chromosome segregation

Zetka, M. C.; Kawasaki, I.; Strome, S.; Muller, F.
Genes & Development – Sep 1, 1999
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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.13.17.2258
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Abstract

Downloaded from genesdev.cshlp.org on November 6, 2021 - Published by Cold Spring Harbor Laboratory Press Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core component that functions in chromosome segregation 1,3 2 2 1 Monique C. Zetka, Ichiro Kawasaki, Susan Strome, and Fritz Mu ¨ ller 1 2 Institute of Zoology, University of Fribourg, Pe ´ rolles, CH-1700 Fribourg, Switzerland; Department of Biology, Indiana University, Bloomington, Indiana 47405 USA Meiotic chromosomes are organized about a proteinaceous core that forms between replicated sister chromatids. We have isolated a Caenorhabditis elegans gene, him-3, which encodes a meiosis-specific component of chromosome cores with some similarity to the yeast lateral element protein Hop1p. Antibodies raised against HIM-3 localize the protein to condensing chromosomes in early prophase I and to the cores of both synapsed and desynapsed chromosomes. In RNA interference experiments, chromosomes appear to condense normally in the absence of detectable protein but fail to synapse and form chiasmata, indicating that HIM-3 is essential for these processes. Hypomorphs of him-3, although being synapsis proficient, show severe reductions in the frequency of crossing-over, demonstrating that HIM-3 has a role in establishing normal levels of interhomolog exchange. Him-3 mutants also show

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Genes & Development – Unpaywall

Published: Sep 1, 1999

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