Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Understanding the role of the cytoskeleton in wood formation in angiosperm trees: hybrid aspen (Populus tremula × P. tremuloides) as the model species

Understanding the role of the cytoskeleton in wood formation in angiosperm trees: hybrid aspen... The involvement of microfilaments and microtubules in the development of the radial and axial components of secondary xylem (wood) in hybrid aspen (Populus tremula L. × P. tremuloidesMichx.) was studied by indirect immunofluorescent localization techniques. In addition to cambial cells, the differentiated cell types considered were early- and late-wood vessel elements, axial parenchyma, normal-wood fibers and gelatinous fibers, and contact and isolation ray cells. Microfilaments were rare in ray cambial cells, but were abundant and axially arranged in their derivatives once cell elongation had begun, and persisted in that orientation in mature ray cells. Microfilaments were axially arranged in fusiform cambial cells and persisted in that orientation in all xylem derivatives of those cells. Microtubules were randomly oriented in ray and fusiform cells of the cambial zone. Dense arrays of parallel-aligned microtubules were oriented near axially in the developing gelatinous fibers, but at a wide range of angles in normal-wood fibers. Ellipses of microfilaments were associated with pit development in fiber cells and isolation ray cells. Rings of co-localized microtubules and microfilaments were associated with developing inter-vessel bordered pits and vessel-contact ray cell contact pits, and, in the case of bordered pits, these rings decreased in diameter as the over-arching pit border increased in size. Although only microtubules were seen at the periphery of the perforation plate of vessel elements, a prominent meshwork of microfilaments overlaid the perforation plate itself. A consensus view of the roles of the cytoskeleton during wood formation in angiosperm trees is presented. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tree Physiology Oxford University Press

Understanding the role of the cytoskeleton in wood formation in angiosperm trees: hybrid aspen (Populus tremula × P. tremuloides) as the model species

Chaffey, Nigel; Barlow, Peter; Sundberg, Björn
Tree Physiology , Volume 22 (4) – Mar 1, 2002
Download PDF
11 pages

Loading next page...
 
/lp/oxford-university-press/understanding-the-role-of-the-cytoskeleton-in-wood-formation-in-47XlsPJBra

References (30)

Publisher
Oxford University Press
Copyright
© Published by Oxford University Press.
ISSN
0829-318X
eISSN
1758-4469
DOI
10.1093/treephys/22.4.239
Publisher site
See Article on Publisher Site

Abstract

The involvement of microfilaments and microtubules in the development of the radial and axial components of secondary xylem (wood) in hybrid aspen (Populus tremula L. × P. tremuloidesMichx.) was studied by indirect immunofluorescent localization techniques. In addition to cambial cells, the differentiated cell types considered were early- and late-wood vessel elements, axial parenchyma, normal-wood fibers and gelatinous fibers, and contact and isolation ray cells. Microfilaments were rare in ray cambial cells, but were abundant and axially arranged in their derivatives once cell elongation had begun, and persisted in that orientation in mature ray cells. Microfilaments were axially arranged in fusiform cambial cells and persisted in that orientation in all xylem derivatives of those cells. Microtubules were randomly oriented in ray and fusiform cells of the cambial zone. Dense arrays of parallel-aligned microtubules were oriented near axially in the developing gelatinous fibers, but at a wide range of angles in normal-wood fibers. Ellipses of microfilaments were associated with pit development in fiber cells and isolation ray cells. Rings of co-localized microtubules and microfilaments were associated with developing inter-vessel bordered pits and vessel-contact ray cell contact pits, and, in the case of bordered pits, these rings decreased in diameter as the over-arching pit border increased in size. Although only microtubules were seen at the periphery of the perforation plate of vessel elements, a prominent meshwork of microfilaments overlaid the perforation plate itself. A consensus view of the roles of the cytoskeleton during wood formation in angiosperm trees is presented.

Journal

Tree Physiology – Oxford University Press

Published: Mar 1, 2002

Keywords: cambium cytodifferentiation immunocytochemistry secondary xylem differentiation tree development

There are no references for this article.