/lp/springer-journals/two-cpg-islands-in-the-sema3b-gene-methylation-in-clear-cell-renal-4eG8FHc07w
References (19)
-
Chris Tse, Ruinua Xiang, Todd Bracht, S. Naylor (2002)
Human Semaphorin 3B (SEMA3B) located at chromosome 3p21.3 suppresses tumor formation in an adenocarcinoma cell line.Cancer research, 62 2
-
L. Hesson, L. Hesson, W. Cooper, F. Latif (2007)
Evaluation of the 3p21.3 tumour-suppressor gene clusterOncogene, 26
-
(2007)
Evaluation of the 3 p 21 . 3 tumoursuppressor gene cluster -
伊藤 正夫 (2006)
Frequent inactivation of RASSF1A, BLU, and SEMA3B on 3p21.3 by promoter hypermethylation and allele loss in non-small cell lung cancer -
J. Herman, J. Graff, S. Myöhänen, B. Nelkin, S. Baylin (1996)
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences of the United States of America, 93 18
-
(2005)
Tumor suppressor genes localized on the short arm of human chromosome -
D. Khodyrev, V. Loginov, I. Pronina, T. Kazubskaya, R. Garkavtseva, E. Braga (2008)
Methylation of promoter region of RAR-β2 gene in renal cell, breast, and ovarian carcinomasRussian Journal of Genetics, 44
-
I. Tischoff, Annett Markwarth, H. Witzigmann, D. Uhlmann, J. Hauss, A. Mirmohammadsadegh, C. Wittekind, U. Hengge, A. Tannapfel (2005)
Allele loss and epigenetic inactivation of 3p21.3 in malignant liver tumorsInternational Journal of Cancer, 115
-
I. Pronina, V. Loginov, V. Prasolov, E. Klimov, D. Khodyrev, T. Kazubskaia, R. Gar’kavtseva, G. Sulimova, E. Braga (2009)
[Alteration of SEMA3B gene expression levels in epithelial tumors].Molekuliarnaia biologiia, 43 3
-
Prakash Nair, L. McArdle, J. Cornell, S. Cohn, R. Stallings (2007)
High-resolution analysis of 3p deletion in neuroblastoma and differential methylation of the SEMA3B tumor suppressor gene.Cancer genetics and cytogenetics, 174 2
-
E. Braga, W. Loginov, E. Klimov, G. Kilosanidze, D. Hodyrev, N. Kaganova, T. Kazubskaya, V. Ermilova, R. Garkavtseva, I. Pronina, O. Rud’ko, E. Zabarovsky, G. Sulimova, L. Kisselev (2006)
Activation of RHOA transcription in epithelial tumors may be caused by gene amplification and/or demethylation of the promoter regionMolecular Biology, 40
-
(2009)
Changes in the level of SEMA3B gene expression in epithelial tumors -
T. Kuroki, F. Trapasso, S. Yendamuri, A. Matsuyama, H. Alder, N. Williams, L. Kaiser, C. Croce (2003)
Allelic loss on chromosome 3p21.3 and promoter hypermethylation of semaphorin 3B in non-small cell lung cancer.Cancer research, 63 12
-
Y. Tomizawa, Y. Sekido, M. Kondo, B. Gao, J. Yokota, J. Roche, H. Drabkin, M. Lerman, A. Gazdar, J. Minna (2001)
Inhibition of lung cancer cell growth and induction of apoptosis after reexpression of 3p21.3 candidate tumor suppressor gene SEMA3BProceedings of the National Academy of Sciences of the United States of America, 98
-
I. Pronina, V. Loginov, V. Prasolov, E. Klimov, D. Khodyrev, T. Kazubskaya, R. Gar’kavtseva, G. Sulimova, E. Braga (2009)
Altered expression of the SEMA3B gene in epithelial tumorsMolecular Biology, 43
-
(2001)
Inhibi - tion of lung cancer cell growth and induction of apoptosis after reexpression of 3 p 21 . 3 candidate tumor suppressor gene SEMA 3 B -
E. Riquelme, M. Tang, S. Báez, Alfonso Díaz, M. Pruyas, I. Wistuba, A. Corvalán (2007)
Frequent epigenetic inactivation of chromosome 3p candidate tumor suppressor genes in gallbladder carcinoma.Cancer letters, 250 1
-
(2005)
Tumor suppressor genes localized on the short arm of human chromosome 3 -
D. Angeloni (2007)
Molecular analysis of deletions in human chromosome 3p21 and the role of resident cancer genes in disease.Briefings in functional genomics & proteomics, 6 1
- Publisher
- Springer Journals
- Copyright
- Copyright © 2009 by Pleiades Publishing, Ltd.
- Subject
- Life Sciences; Human Genetics ; Biochemistry, general; Life Sciences, general
- ISSN
- 0026-8933
- eISSN
- 1608-3245
- DOI
- 10.1134/S0026893309060156
- Publisher site
- See Article on Publisher Site
Abstract
Earlier, methylation of a CpG island in the SEMA3B gene (3p21.31) was observed in cell lines of small-cell and non-small-cell lung carcinoma. According to NCBI (Build 36), that island belonged to intron 1 of the gene. Our study concerns the methylation of two CpG islands, promoter and intronic, in the SEMA3B gene in patients with clear cell renal cell carcinoma (RCC). Methylation-specific PCR and bisulfite sequencing revealed a high frequency of methylation in the promoter CpG island (34/61, 56%) and somewhat lower, in the intronic (17/48, 35%). A significant inverse correlation was found between the SEMA3B mRNA level and methylation of the promoter CpG island in RCC (P < 0.05 according to Fisher’s exact test). The intronic island showed no such correlation. Thus, we suggest that the methylation of the promoter CpG island contributes to the inactivation of the SEMA3B suppressor gene in RCC tissue.
Journal
Molecular Biology – Springer Journals
Published: Dec 4, 2009