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Sequence of a cDNA coding for human IRF-1

Sequence of a cDNA coding for human IRF-1 Downloaded from https://academic.oup.com/nar/article/17/8/3292/2383255 by DeepDyve user on 14 August 2020 volume 17 Number 8 1989 Nucleic Acids Research Mitsuo Maruyama. Takashi Fujita and Tadatsugu Taniguchi* Institute for Molecular and Cellular Biology, Osaka University, Yamadaoka Suila-shi, Osaka 565, Japan Submitted March 17. 1989 EMBL accession no. X14454 We have previously isolated and sequenced a cDNA clone encoding a mouse IFN regulatory factor 1 (IRF-1) (Miyamoto, M., Fujita, T., Kimura, Y., Maruyama, M., Harada, H., Sudo, Y., Miyata, T., and Taniguchi, T., 1988, Cell 54., 903-913). We have isolated a cDNA coding for human IRF-1 by cross-hybridization with the A. 28-8-derived mouse cDNA probe, using the Human T cell line, Jurkat-111 cDNA library. One of the positive clones, pHIRF31, contained the largest cDNA insert and its nucleotide sequence was determined. The cDNA includes 197bp of the 5'- untranslated, 975bp coding and 863bp 3'-untranslated regions. The deduced amino acid sequence of the 325 amino acid protein, shows 83% homology with mouse IRF-1; The amino- terminal half, spanning from amino acid residues 1 to 140 are highly conserved (95%) in the two species. 1 CGAGCCCCGCCGAACCGAGGCCACCCGGAGCCGTGCCCAGTCCACGCCGGCCGTGCCCGGCGGCCTTAAGAACCAGGCAACCTCTGCCTTCTTCCCTC 198 ATG CCC ATC ACT TGG ATG CGC ATG AGA CCC TGG CTA GAG ATG CAG ATT AAT TCC AAC CAA ATC CCG GGG CTC ATC Met Pro H e Thr Trp Met Arg Met Arg Pro Trp Leu Glu Met Gin lie Aan Ser Aan Gin H e Pro Gly Leu H e 273 TGG ATT AAT AAA GAG GAG ATG ATC TTG GAG ATC CCA TGG AAG CAT GCT OCC AAG CAT GGC TGG GAC ATC AAC AAG Trp H e Asn Lys Glu Glu Met lie Leu Glu H e Pro Trp Lys His Ala Ala Lys His Gly Trp Asp H e Asn Lys 348 GAT GCC TGT TTG TTC CGG AGC TGG GCC ATT CAC ACA GGC CGA TAC AAA GCA GGG GAA AAG GAG CCA GAT CCC AAG Asp Ala Cyfl Leu Phe Arg Ser Trp Ala H e His Thr Gly Arg Tyr Lys Ala Gly Glu Lys Glu Pro Asp pro Lys 423 ACG TGG AAG GCC AAC TTT CGC TGT GCC ATG AAC TCC CTG CCA GAT ATC GAG GAG GTG AAA GAC CAG AGC AGG AAC Thr Trp Lys Ala Asn Phe Arg Cya Ala Met Asn Ser Leu Pro Asp H e Glu Glu Val Lys Asp Gin Ser Arg Asn 498 AAG GGC AGC TCA GCT GTG CGA GTG TAC CGG ATG CTT CCA CCT CTC ACC AAG AAC CAG AGA AAA GAA AGA AAG TCG Lys Gly Ser Ser Ala Val Arg val Tyr Arg Met Leu pro Pro Leu Thr Lys Asn Gin Arg Lys Glu Arg Lys Ser 573 AAG TCC AGC CGA GAT GCT AAG AGC AAG GCC AAG AGG AAG TCA TGT GGG GAT TCC AGC CCT GAT ACC TTC TCT GAT Lys Ser Ser Arg Asp Ala Lys Ser Lye Ala Lya Arg Lys Ser Cys Gly AB P Ser Ser Pro Asp Thr Phe Ser Asp 648 GGA CTC AGC AGC TCC ACT CTG CCT GAT GAC CAC AGC AGC TAC ACA GTT CCA GGC TAC ATG CAG GAC TTG GAG GTG Gly Leu Ser Ser Ser Thr Leu Pro Asp Asp His Ser Ser Tyr Thr Val Pro Gly Tyr Met Gin Asp Leu Glu Val 723 GAG CAG GCC CTG ACT CCA GCA CTG TCG CCA TGT GCT GTC AGC AGC ACT CTC CCC GAC TGG CAC ATC CCA GTG GAA Glu Gin Ala Leu Thr Pro Ala Leu Ser Pro Cys Ala Val Ser Ser Thr Leu Pro Asp Trp HI B H e Pro val Glu 798 GTT GTG CCG GAC AGC ACC AGT GAT CTG TAC AAC TTC CAG GTG TCA CCC ATG CCC TCC ATC TCT GAA GCT ACA ACA Val Val Pro Asp Ser Thr Ser Asp Leu Tyr Asn Phe Gin val Ser Pro Met Pro Ser H e Ser Glu Ala Thr Thr 873 GAT GAG GAT GAG GAA GGG AAA TTA CCT GAG GAC ATC ATG AAG CTC TTG GAG CAG TCG GAG TGG CAG CCA ACA AAC Asp Glu Asp Glu Glu Gly Lys Leu Pro Glu Asp H e Met Lys Leu Leu Glu Gin Ser Glu Trp Gin Pro Thr Asn 948 GTG GAT GGG AAG GGG TAC CTA CTC AAT GAA CCT GGA GTC CAG CCC ACC TCT GTC TAT GGA GAC TTT AGC TGT AAG Val Asp Gly Lys Gly Tyr Leu Leu Asn Glu Pro Gly val Gin pro Thr Ser Val Tyr Gly Asp Phe Ser CyB Lys 1023 GAG GAG CCA GAA ATT GAC AGC CCA GGG GGG GAT ATT GGG CTG AGT CTA CAG CGT GTC TTC ACA GAT CTG AAG AAC Glu Glu Pro Glu H e Asp Ser Pro Gly Gly Asp H e Gly Leu Ser Leu Gin Arg Val Phe Thr Asp Leu Lys Asn 1098 ATG GAT GCC ACC TGG CTG GAC AGC CTG CTG ACC CCA GTC CGG TTG CCC TCC ATC CAG GCC ATT CCC TGT GCA CCG Met Asp Ala Thr Trp Leu Aep Ser Leu Leu Thr Pro val Arg Leu Pro Ser H e Gin Ala H e Pro Cys Ala Pro 1173 TAG CAGGGCCCCTGO5CCCCTCTTATTCCTCTAGGCAAGCAGGACCTGGCATCATGGTGGATATGGTG(»GAGAAGCTGGACTTCTGTGGGCCCCTCA 1271 ACAGCCAAGTGTGACCCC»CTGCCAAGTGGG<»TGGGCCTCCCTCCTTGGGT(»TTGACCTCTCAGGGCCTGGCAGGCCAGTGTCTGGGlTTTTCrTGT 1370 CGTGTAAAGCTGGCCCTGCCTCCTGGGAAGATGAGGTTCTGAGACCAGTGTATCAGGTCAGGGACTTGGA 1568 CTG«CTAAGCGAAAATTGCACTAAATGAATCT(XnTCCAAAGAACTACCCCTTTTCAGCTC^ 1667 GAAACTCCCCTCCTTCGGGGCMTGCTCCCTOulCCTCAGAGGAGCTCTACCCTGCTCCCTGCT 1766 TTTCGTGTGGATCTTGCCACATTTCTGATCAGAGGTGTACACTAACATTTCCCCCGAGCTCTTGGCCTTTGMTTTATTTATACAGTGCCTTC 1865 GCCCACCACCCCCTCAAGCCCCAG(»GCCCTCAACAGGCCCAGGGAGGGAAGTCTGAGCGCCTTGGTATGACTTAAAATTGGAAATGTCATCTAACCAT 1964 TJUU3TCATGTGTGAACACATAAGGACGTGTGTAJULTATGTACATTTGTCTTTTTATAAAAAGTAAAATTGTT 2035 *To whom correspondence should be addressed 3292 ©IRLPress http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nucleic Acids Research Oxford University Press

Sequence of a cDNA coding for human IRF-1

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Oxford University Press
Copyright
© IRL Press
ISSN
0305-1048
eISSN
1362-4962
DOI
10.1093/nar/17.8.3292
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Abstract

Downloaded from https://academic.oup.com/nar/article/17/8/3292/2383255 by DeepDyve user on 14 August 2020 volume 17 Number 8 1989 Nucleic Acids Research Mitsuo Maruyama. Takashi Fujita and Tadatsugu Taniguchi* Institute for Molecular and Cellular Biology, Osaka University, Yamadaoka Suila-shi, Osaka 565, Japan Submitted March 17. 1989 EMBL accession no. X14454 We have previously isolated and sequenced a cDNA clone encoding a mouse IFN regulatory factor 1 (IRF-1) (Miyamoto, M., Fujita, T., Kimura, Y., Maruyama, M., Harada, H., Sudo, Y., Miyata, T., and Taniguchi, T., 1988, Cell 54., 903-913). We have isolated a cDNA coding for human IRF-1 by cross-hybridization with the A. 28-8-derived mouse cDNA probe, using the Human T cell line, Jurkat-111 cDNA library. One of the positive clones, pHIRF31, contained the largest cDNA insert and its nucleotide sequence was determined. The cDNA includes 197bp of the 5'- untranslated, 975bp coding and 863bp 3'-untranslated regions. The deduced amino acid sequence of the 325 amino acid protein, shows 83% homology with mouse IRF-1; The amino- terminal half, spanning from amino acid residues 1 to 140 are highly conserved (95%) in the two species. 1 CGAGCCCCGCCGAACCGAGGCCACCCGGAGCCGTGCCCAGTCCACGCCGGCCGTGCCCGGCGGCCTTAAGAACCAGGCAACCTCTGCCTTCTTCCCTC 198 ATG CCC ATC ACT TGG ATG CGC ATG AGA CCC TGG CTA GAG ATG CAG ATT AAT TCC AAC CAA ATC CCG GGG CTC ATC Met Pro H e Thr Trp Met Arg Met Arg Pro Trp Leu Glu Met Gin lie Aan Ser Aan Gin H e Pro Gly Leu H e 273 TGG ATT AAT AAA GAG GAG ATG ATC TTG GAG ATC CCA TGG AAG CAT GCT OCC AAG CAT GGC TGG GAC ATC AAC AAG Trp H e Asn Lys Glu Glu Met lie Leu Glu H e Pro Trp Lys His Ala Ala Lys His Gly Trp Asp H e Asn Lys 348 GAT GCC TGT TTG TTC CGG AGC TGG GCC ATT CAC ACA GGC CGA TAC AAA GCA GGG GAA AAG GAG CCA GAT CCC AAG Asp Ala Cyfl Leu Phe Arg Ser Trp Ala H e His Thr Gly Arg Tyr Lys Ala Gly Glu Lys Glu Pro Asp pro Lys 423 ACG TGG AAG GCC AAC TTT CGC TGT GCC ATG AAC TCC CTG CCA GAT ATC GAG GAG GTG AAA GAC CAG AGC AGG AAC Thr Trp Lys Ala Asn Phe Arg Cya Ala Met Asn Ser Leu Pro Asp H e Glu Glu Val Lys Asp Gin Ser Arg Asn 498 AAG GGC AGC TCA GCT GTG CGA GTG TAC CGG ATG CTT CCA CCT CTC ACC AAG AAC CAG AGA AAA GAA AGA AAG TCG Lys Gly Ser Ser Ala Val Arg val Tyr Arg Met Leu pro Pro Leu Thr Lys Asn Gin Arg Lys Glu Arg Lys Ser 573 AAG TCC AGC CGA GAT GCT AAG AGC AAG GCC AAG AGG AAG TCA TGT GGG GAT TCC AGC CCT GAT ACC TTC TCT GAT Lys Ser Ser Arg Asp Ala Lys Ser Lye Ala Lya Arg Lys Ser Cys Gly AB P Ser Ser Pro Asp Thr Phe Ser Asp 648 GGA CTC AGC AGC TCC ACT CTG CCT GAT GAC CAC AGC AGC TAC ACA GTT CCA GGC TAC ATG CAG GAC TTG GAG GTG Gly Leu Ser Ser Ser Thr Leu Pro Asp Asp His Ser Ser Tyr Thr Val Pro Gly Tyr Met Gin Asp Leu Glu Val 723 GAG CAG GCC CTG ACT CCA GCA CTG TCG CCA TGT GCT GTC AGC AGC ACT CTC CCC GAC TGG CAC ATC CCA GTG GAA Glu Gin Ala Leu Thr Pro Ala Leu Ser Pro Cys Ala Val Ser Ser Thr Leu Pro Asp Trp HI B H e Pro val Glu 798 GTT GTG CCG GAC AGC ACC AGT GAT CTG TAC AAC TTC CAG GTG TCA CCC ATG CCC TCC ATC TCT GAA GCT ACA ACA Val Val Pro Asp Ser Thr Ser Asp Leu Tyr Asn Phe Gin val Ser Pro Met Pro Ser H e Ser Glu Ala Thr Thr 873 GAT GAG GAT GAG GAA GGG AAA TTA CCT GAG GAC ATC ATG AAG CTC TTG GAG CAG TCG GAG TGG CAG CCA ACA AAC Asp Glu Asp Glu Glu Gly Lys Leu Pro Glu Asp H e Met Lys Leu Leu Glu Gin Ser Glu Trp Gin Pro Thr Asn 948 GTG GAT GGG AAG GGG TAC CTA CTC AAT GAA CCT GGA GTC CAG CCC ACC TCT GTC TAT GGA GAC TTT AGC TGT AAG Val Asp Gly Lys Gly Tyr Leu Leu Asn Glu Pro Gly val Gin pro Thr Ser Val Tyr Gly Asp Phe Ser CyB Lys 1023 GAG GAG CCA GAA ATT GAC AGC CCA GGG GGG GAT ATT GGG CTG AGT CTA CAG CGT GTC TTC ACA GAT CTG AAG AAC Glu Glu Pro Glu H e Asp Ser Pro Gly Gly Asp H e Gly Leu Ser Leu Gin Arg Val Phe Thr Asp Leu Lys Asn 1098 ATG GAT GCC ACC TGG CTG GAC AGC CTG CTG ACC CCA GTC CGG TTG CCC TCC ATC CAG GCC ATT CCC TGT GCA CCG Met Asp Ala Thr Trp Leu Aep Ser Leu Leu Thr Pro val Arg Leu Pro Ser H e Gin Ala H e Pro Cys Ala Pro 1173 TAG CAGGGCCCCTGO5CCCCTCTTATTCCTCTAGGCAAGCAGGACCTGGCATCATGGTGGATATGGTG(»GAGAAGCTGGACTTCTGTGGGCCCCTCA 1271 ACAGCCAAGTGTGACCCC»CTGCCAAGTGGG<»TGGGCCTCCCTCCTTGGGT(»TTGACCTCTCAGGGCCTGGCAGGCCAGTGTCTGGGlTTTTCrTGT 1370 CGTGTAAAGCTGGCCCTGCCTCCTGGGAAGATGAGGTTCTGAGACCAGTGTATCAGGTCAGGGACTTGGA 1568 CTG«CTAAGCGAAAATTGCACTAAATGAATCT(XnTCCAAAGAACTACCCCTTTTCAGCTC^ 1667 GAAACTCCCCTCCTTCGGGGCMTGCTCCCTOulCCTCAGAGGAGCTCTACCCTGCTCCCTGCT 1766 TTTCGTGTGGATCTTGCCACATTTCTGATCAGAGGTGTACACTAACATTTCCCCCGAGCTCTTGGCCTTTGMTTTATTTATACAGTGCCTTC 1865 GCCCACCACCCCCTCAAGCCCCAG(»GCCCTCAACAGGCCCAGGGAGGGAAGTCTGAGCGCCTTGGTATGACTTAAAATTGGAAATGTCATCTAACCAT 1964 TJUU3TCATGTGTGAACACATAAGGACGTGTGTAJULTATGTACATTTGTCTTTTTATAAAAAGTAAAATTGTT 2035 *To whom correspondence should be addressed 3292 ©IRLPress

Journal

Nucleic Acids Research – Oxford University Press

Published: Apr 25, 1989

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