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Prevention by melatonin of hepatocarcinogenesis in rats injected with N ‐nitrosodiethylamine

Prevention by melatonin of hepatocarcinogenesis in rats injected with N ‐nitrosodiethylamine Abstract: N‐nitrosodiethylamine (NDEA) is a potent carcinogenic agent that induces liver cancer. To evaluate the chemopreventive function of melatonin in this experimental model, Wistar male rats received a single i.p. injection of NDEA or vehicle followed by weekly s.c. injections of carbon tetrachloride or vehicle for 6 weeks. Melatonin (5 mg/kg body weight) or its vehicle (0.5 mL saline) was given i.p. on a daily basis 2 hr before lights off for 20 wk. At the end of this period the rats were killed and liver and blood samples were taken for histological and biochemical studies. As markers for liver function, the activity of aspartate transaminase (AST) and alanine transaminase (ALT) and the levels of α‐fetoprotein were measured in serum. To assess lipid peroxidation and the antioxidant status in liver and blood, the levels of thiobarbituric acid reactive substances (TBARS) and of reduced glutathione (GSH) were measured. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S‐transferase (GST) was assessed in liver and erythrocyte fraction of NDEA‐treated rats. NDEA administration inhibited body weight, macro‐ and microscopically detectable liver tumors and increased levels of plasma AST, ALT and α‐fetoprotein. NDEA treatment decreased liver TBARS levels and CAT and SOD activities and increased liver GSH levels and GST and GPx activities. Plasma TBARS were augmented, while plasma GSH levels and the activities of erythrocyte CAT, SOD, GST and GPx decreased, in NDEA‐treated rats. Melatonin administration significantly curtailed tumor development and counteracted all the biochemical effects. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Pineal Research Wiley

Prevention by melatonin of hepatocarcinogenesis in rats injected with N ‐nitrosodiethylamine

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References (49)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0742-3098
eISSN
1600-079X
DOI
10.1111/j.1600-079X.2007.00478.x
pmid
17803529
Publisher site
See Article on Publisher Site

Abstract

Abstract: N‐nitrosodiethylamine (NDEA) is a potent carcinogenic agent that induces liver cancer. To evaluate the chemopreventive function of melatonin in this experimental model, Wistar male rats received a single i.p. injection of NDEA or vehicle followed by weekly s.c. injections of carbon tetrachloride or vehicle for 6 weeks. Melatonin (5 mg/kg body weight) or its vehicle (0.5 mL saline) was given i.p. on a daily basis 2 hr before lights off for 20 wk. At the end of this period the rats were killed and liver and blood samples were taken for histological and biochemical studies. As markers for liver function, the activity of aspartate transaminase (AST) and alanine transaminase (ALT) and the levels of α‐fetoprotein were measured in serum. To assess lipid peroxidation and the antioxidant status in liver and blood, the levels of thiobarbituric acid reactive substances (TBARS) and of reduced glutathione (GSH) were measured. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S‐transferase (GST) was assessed in liver and erythrocyte fraction of NDEA‐treated rats. NDEA administration inhibited body weight, macro‐ and microscopically detectable liver tumors and increased levels of plasma AST, ALT and α‐fetoprotein. NDEA treatment decreased liver TBARS levels and CAT and SOD activities and increased liver GSH levels and GST and GPx activities. Plasma TBARS were augmented, while plasma GSH levels and the activities of erythrocyte CAT, SOD, GST and GPx decreased, in NDEA‐treated rats. Melatonin administration significantly curtailed tumor development and counteracted all the biochemical effects.

Journal

Journal of Pineal Research – Wiley

Published: Oct 1, 2007

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