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Uptake and Autoreceptor-Controlled Release of [3H]-GABA by the Hypothalamic Median Eminence and Pituitary Neurointermediate Lobe

Uptake and Autoreceptor-Controlled Release of [3H]-GABA by the Hypothalamic Median Eminence and... The uptake and release of γ-[<sup>3</sup>H]-aminobutyric acid ([<sup>3</sup>H]-GABA) by the median eminence and the neurointermediate lobe of the pituitary was investigated using sucrose homogenates as crude synaptosomal preparations. Uptake in both areas showed predominantly neuronal specificity and similar K<sub>m</sub> values, but the median eminence had a considerably greater V<sub>max</sub> value. Release of [<sup>3</sup>H]-GABA could be stimulated by elevated K<sup>+</sup> concentrations, in a Ca<sup>2+</sup>-dependent manner. Stimulated release was reduced by muscimol, implying the existence of presynaptic autoreceptors in both areas. A number of other transmitters and peptide hormones were demonstrated to have no effect on stimulated release of [<sup>3</sup>H] GABA in either area. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroendocrinology Karger

Uptake and Autoreceptor-Controlled Release of [3H]-GABA by the Hypothalamic Median Eminence and Pituitary Neurointermediate Lobe

Anderson, Richard; Mitchell, Rory
Neuroendocrinology , Volume 42 (4): 8 – Jan 1, 1986
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Publisher
Karger
Copyright
© 1986 S. Karger AG, Basel
ISSN
0028-3835
eISSN
1423-0194
DOI
10.1159/000124452
Publisher site
See Article on Publisher Site

Abstract

The uptake and release of γ-[<sup>3</sup>H]-aminobutyric acid ([<sup>3</sup>H]-GABA) by the median eminence and the neurointermediate lobe of the pituitary was investigated using sucrose homogenates as crude synaptosomal preparations. Uptake in both areas showed predominantly neuronal specificity and similar K<sub>m</sub> values, but the median eminence had a considerably greater V<sub>max</sub> value. Release of [<sup>3</sup>H]-GABA could be stimulated by elevated K<sup>+</sup> concentrations, in a Ca<sup>2+</sup>-dependent manner. Stimulated release was reduced by muscimol, implying the existence of presynaptic autoreceptors in both areas. A number of other transmitters and peptide hormones were demonstrated to have no effect on stimulated release of [<sup>3</sup>H] GABA in either area.

Journal

Neuroendocrinology – Karger

Published: Jan 1, 1986

Keywords: γ-Aminobutyric acid uptake; γ-Aminobutyric acid release; Median eminence; Neurointermediate lobe

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