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Development and Validation of a radioimmunoassay for human secretory ‘pregnancy-associated endometrial α2-globulin’ (α2-PEG) and detection in serum during pregnancy

Development and Validation of a radioimmunoassay for human secretory ‘pregnancy-associated... Abstract A specific and sensitive radioimmunoassay has been developed for pregnancy-associated endometrial α2-globulin (α2-PEG), the major secretory protein of the human endometrium during the mid- to lateluteal phase of the menstrual cycle and first trimester of pregnancy. This assay enabled α2-PEG to be measured in the cytosolic extracts of endometrium, am-niotic fluid, seminal plasma and in pregnancy sera. The concentration of aλPEG in cytosols prepared from mid-secretory endometrium was 44-fold higher than in those from proliferative endometrium and a further 30-fold increase during the first trimester in comparison with mid-secretory endometrium. Levels of α2-PEG in amniotk fluid (15-20 weeks) and seminal plasma were 15 ± 4 and 55 ± 7 /tg/ml, respectively. With the exception of sera, where α2-PEG was undetectable using rocket immunoelectrophoresis, estimates of arPEG levels in these compartments, measured by rocket immunoelectrophoretic and RIA assays were comparable, although for seminal plasma 2.5-fold higher estimates were obtained by RIA. Detectable levels of a2-PEG were obtained in 253 of 275 (927percnt;) pregnancy sera tested. During pregnancy, peak levels were detected between weeks 6 and 11 but these represented only 2% of the levels detected in amniotk fluid. These observations support evidence from in-vitro studies that a2-PEG is a product of the secretory glandular epithelium, a tissue prominent in the first trimester and whose principal secretory route during pregnancy is into the amniotic fluid. The potential clinical use of serum α2-PEG measurements and the possible function of α2-PEG in pregnancy is discussed. Endometrium, glandular epithelium This content is only available as a PDF. © 1987 IRL Press Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Reproduction Oxford University Press

Development and Validation of a radioimmunoassay for human secretory ‘pregnancy-associated endometrial α2-globulin’ (α2-PEG) and detection in serum during pregnancy

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Publisher
Oxford University Press
Copyright
© 1987 IRL Press Ltd.
ISSN
0268-1161
eISSN
1460-2350
DOI
10.1093/oxfordjournals.humrep.a136556
Publisher site
See Article on Publisher Site

Abstract

Abstract A specific and sensitive radioimmunoassay has been developed for pregnancy-associated endometrial α2-globulin (α2-PEG), the major secretory protein of the human endometrium during the mid- to lateluteal phase of the menstrual cycle and first trimester of pregnancy. This assay enabled α2-PEG to be measured in the cytosolic extracts of endometrium, am-niotic fluid, seminal plasma and in pregnancy sera. The concentration of aλPEG in cytosols prepared from mid-secretory endometrium was 44-fold higher than in those from proliferative endometrium and a further 30-fold increase during the first trimester in comparison with mid-secretory endometrium. Levels of α2-PEG in amniotk fluid (15-20 weeks) and seminal plasma were 15 ± 4 and 55 ± 7 /tg/ml, respectively. With the exception of sera, where α2-PEG was undetectable using rocket immunoelectrophoresis, estimates of arPEG levels in these compartments, measured by rocket immunoelectrophoretic and RIA assays were comparable, although for seminal plasma 2.5-fold higher estimates were obtained by RIA. Detectable levels of a2-PEG were obtained in 253 of 275 (927percnt;) pregnancy sera tested. During pregnancy, peak levels were detected between weeks 6 and 11 but these represented only 2% of the levels detected in amniotk fluid. These observations support evidence from in-vitro studies that a2-PEG is a product of the secretory glandular epithelium, a tissue prominent in the first trimester and whose principal secretory route during pregnancy is into the amniotic fluid. The potential clinical use of serum α2-PEG measurements and the possible function of α2-PEG in pregnancy is discussed. Endometrium, glandular epithelium This content is only available as a PDF. © 1987 IRL Press Ltd.

Journal

Human Reproduction – Oxford University Press

Published: Jul 1, 1987

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