Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Transposase is the only nematode protein required for in vitro transposition of Tc1.

Transposase is the only nematode protein required for in vitro transposition of Tc1. Downloaded from genesdev.cshlp.org on October 8, 2021 - Published by Cold Spring Harbor Laboratory Press Transposase.is the only nematode protein required for in vitro transposition of Tcl Jan c. Vos, Ivo De Baere, and Ronald H.A. Plasterk 1 The Netherlands Cancer Institute, Division of Molecular Biology, 1066 CX Amsterdam, Netherlands The Tcl element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tcl in a cell-free system. Tcl appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tcl terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in vitro is similar to that in vivo. Transposition is achieved with an extract prepared from nuclei of transgenic nematodes that overexpress Tcl transposase but also by recombinant transposase purified from Escherichia coli. The simple reaction requirements explain why horizontal spread of Tcl/mariner transposons can occur. They also suggest that Tcl may be a good vector for transgenesis of diverse animal species. [Key Words: C. elegans; in vitro transposition; Tcl; transgenesis] Received December 18, 1995; revised version accepted January 29, 1996. Tcl belongs http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Transposase is the only nematode protein required for in vitro transposition of Tc1.

Vos, J C; De Baere, I; Plasterk, R H
Genes & Development – Mar 15, 1996
Download PDF
8 pages

Loading next page...
 
/lp/unpaywall/transposase-is-the-only-nematode-protein-required-for-in-vitro-9w6eHYpft4

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.10.6.755
Publisher site
See Article on Publisher Site

Abstract

Downloaded from genesdev.cshlp.org on October 8, 2021 - Published by Cold Spring Harbor Laboratory Press Transposase.is the only nematode protein required for in vitro transposition of Tcl Jan c. Vos, Ivo De Baere, and Ronald H.A. Plasterk 1 The Netherlands Cancer Institute, Division of Molecular Biology, 1066 CX Amsterdam, Netherlands The Tcl element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tcl in a cell-free system. Tcl appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tcl terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in vitro is similar to that in vivo. Transposition is achieved with an extract prepared from nuclei of transgenic nematodes that overexpress Tcl transposase but also by recombinant transposase purified from Escherichia coli. The simple reaction requirements explain why horizontal spread of Tcl/mariner transposons can occur. They also suggest that Tcl may be a good vector for transgenesis of diverse animal species. [Key Words: C. elegans; in vitro transposition; Tcl; transgenesis] Received December 18, 1995; revised version accepted January 29, 1996. Tcl belongs

Journal

Genes & Development – Unpaywall

Published: Mar 15, 1996

There are no references for this article.