Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Depolarizing stimulation upregulates GA‐binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits

Depolarizing stimulation upregulates GA‐binding protein in neurons: a transcription factor... Neurons are unique in having dendrites that extend far away from their cell bodies. Mitochondria located in the dendrites can be separated from the nucleus for long distances. The mechanism of bigenomic coordination is of particular importance to cytochrome oxidase (CO), which has subunits that are encoded in both the nuclear and mitochondrial DNA. GA‐binding protein (GABP) is a transcription factor that is required for the promoter activity of mitochondrial transcription factor A as well as several nuclear‐encoded CO subunits. Thus, GABP may play a key role in coordinating the transcription of both mitochondrial and nuclear‐encoded subunits of CO. The goal of the present study was to determine if GABP was expressed in neurons and whether and how it responded to increased neuronal activity. Using primary neuronal cultures, the β‐subunit of GABP was localized immunocytochemically to both the cytoplasm and the nucleus, whereas the α‐subunit was expressed mainly in the nucleus. In KCl‐treated cultures, immunoreactivity for both α‐ and β‐subunits was significantly increased in the nucleus compared with untreated sister cultures. The induction of GABP preceded that of CO gene expression from the two genomes, which, in turn, preceded that of CO activity. Thus, our data suggest that neuronal activity regulates subunit concentrations of GABP in the nucleus, and GABP may be a critical sensor of changes in neuronal activity. Our data are also consistent with the postulated role of GABP as a coordinator of both mitochondrial and nuclear transcription for subunits of CO in neurons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Depolarizing stimulation upregulates GA‐binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits

Download PDF
11 pages

Loading next page...
 
/lp/wiley/depolarizing-stimulation-upregulates-ga-binding-protein-in-neurons-a-B0jvs8SssO

References (67)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1046/j.1460-9568.2000.00997.x
Publisher site
See Article on Publisher Site

Abstract

Neurons are unique in having dendrites that extend far away from their cell bodies. Mitochondria located in the dendrites can be separated from the nucleus for long distances. The mechanism of bigenomic coordination is of particular importance to cytochrome oxidase (CO), which has subunits that are encoded in both the nuclear and mitochondrial DNA. GA‐binding protein (GABP) is a transcription factor that is required for the promoter activity of mitochondrial transcription factor A as well as several nuclear‐encoded CO subunits. Thus, GABP may play a key role in coordinating the transcription of both mitochondrial and nuclear‐encoded subunits of CO. The goal of the present study was to determine if GABP was expressed in neurons and whether and how it responded to increased neuronal activity. Using primary neuronal cultures, the β‐subunit of GABP was localized immunocytochemically to both the cytoplasm and the nucleus, whereas the α‐subunit was expressed mainly in the nucleus. In KCl‐treated cultures, immunoreactivity for both α‐ and β‐subunits was significantly increased in the nucleus compared with untreated sister cultures. The induction of GABP preceded that of CO gene expression from the two genomes, which, in turn, preceded that of CO activity. Thus, our data suggest that neuronal activity regulates subunit concentrations of GABP in the nucleus, and GABP may be a critical sensor of changes in neuronal activity. Our data are also consistent with the postulated role of GABP as a coordinator of both mitochondrial and nuclear transcription for subunits of CO in neurons.

Journal

European Journal of Neuroscience – Wiley

Published: Mar 1, 2000

There are no references for this article.