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Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence

Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence A highly specific antisera was produced in New Zealand white rabbits against nisin Z, a 3400 Da bacteriocin produced by Lactococcus lactis ssp. lactis biovar. diacetylactis UL 719. A dot immunoblot assay was then developed to detect nisin Z in milk and whey. As few as 1·5 10−1 international units per ml (IU ml−1), corresponding to 0·003 μg ml−1 of pure nisin Z, were detected in carbonate‐bicarbonate buffer within 6 h using chemiluminescence. When milk and whey samples were tested, approximately 0·155 μg ml−1 (7·9 IU ml−1) of nisin Z was detected. The detection limit obtained was lower than that of traditional methods including microtitration and agar diffusion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Oxford University Press

Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence

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References (28)

Publisher
Oxford University Press
Copyright
Society for Applied Microbiology
ISSN
1364-5072
eISSN
1365-2672
DOI
10.1046/j.1365-2672.1998.00315.x
Publisher site
See Article on Publisher Site

Abstract

A highly specific antisera was produced in New Zealand white rabbits against nisin Z, a 3400 Da bacteriocin produced by Lactococcus lactis ssp. lactis biovar. diacetylactis UL 719. A dot immunoblot assay was then developed to detect nisin Z in milk and whey. As few as 1·5 10−1 international units per ml (IU ml−1), corresponding to 0·003 μg ml−1 of pure nisin Z, were detected in carbonate‐bicarbonate buffer within 6 h using chemiluminescence. When milk and whey samples were tested, approximately 0·155 μg ml−1 (7·9 IU ml−1) of nisin Z was detected. The detection limit obtained was lower than that of traditional methods including microtitration and agar diffusion.

Journal

Journal of Applied Microbiology – Oxford University Press

Published: Feb 1, 1998

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