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Human Cell Culture ProtocolsHuman Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation

Human Cell Culture Protocols: Human Chondrocyte Cultures as Models of Cartilage-Specific Gene... [Chondrocytes comprise the single cellular component of adult hyaline cartilage and are considered to be terminally differentiated cells that maintain the cartilage matrix when turnover is low. The major components of the extracellular matrix synthesized by these specialized cells include highly crosslinked fibrils of the final synthesized and secreted triple helical type II collagen molecule that interact with other cartilage-specific collagens IX and XI, the large aggregating proteoglycan aggrecan, small proteoglycans, such as biglycan and decorin, and other specific and nonspecific matrix proteins that are expressed at defined stages during development and growth 1, 2. This matrix confers tensile strength and flexibility to articular surfaces and serves specialized functions in only a few other tissues Cultured chondrocytes have served as useful models for studying chondrocyte differentiation and the effects of cytokines and growth factors that control the maintenance or suppression of differentiated cartilage phenotype 3.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Human Cell Culture ProtocolsHuman Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation

Part of the Methods in Molecular Medicine Book Series (volume 2)
Editors: Jones, Gareth E.
Goldring, Mary B.
Human Cell Culture Protocols — Jan 1, 1996
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16 pages

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References (35)

Publisher
Humana Press
Copyright
© Humana Press 1996
ISBN
978-0-89603-335-1
Pages
217–232
DOI
10.1385/0-89603-335-X:217
Publisher site
See Chapter on Publisher Site

Abstract

[Chondrocytes comprise the single cellular component of adult hyaline cartilage and are considered to be terminally differentiated cells that maintain the cartilage matrix when turnover is low. The major components of the extracellular matrix synthesized by these specialized cells include highly crosslinked fibrils of the final synthesized and secreted triple helical type II collagen molecule that interact with other cartilage-specific collagens IX and XI, the large aggregating proteoglycan aggrecan, small proteoglycans, such as biglycan and decorin, and other specific and nonspecific matrix proteins that are expressed at defined stages during development and growth 1, 2. This matrix confers tensile strength and flexibility to articular surfaces and serves specialized functions in only a few other tissues Cultured chondrocytes have served as useful models for studying chondrocyte differentiation and the effects of cytokines and growth factors that control the maintenance or suppression of differentiated cartilage phenotype 3.]

Published: Jan 1, 1996

Keywords: Costal Cartilage; Gene Regulatory Sequence; Alginate Culture; Costal Chondrocytes; Human Chondrosarcoma Cell Line

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