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OpenFovea: open-source AFM data processing software

OpenFovea: open-source AFM data processing software No. not event correspond ence and Asylum Research (.ibw files). Support for Park Systems micro- OpenFovea: open-source AFM data scopes is under development. Images and graphs generated using OpenFovea can be saved as processing software bitmaps (.jpg, .png) or as vector images (.pdf, .svg). Raw data can To the Editor: The atomic force microscope (AFM) is often used be exported as comma-separated values that can be imported into to detect specific molecules on the surfaces of specimens and to spreadsheets or statistical software. OpenFovea is capable of saving measure a specimen’s mechanical properties . This information can the original force-distance curves as well as the computed param- be deduced from the force-distance curves generated by the deflec- eters and results. tion of the cantilever as it approaches, indents and recedes from To demonstrate the processing and display capabilities of our soft- the sample. Force-volume images can be generated from a series of ware, we used OpenFovea to process data collected in force-volume successive force-distance curves recorded while scanning an area imaging mode on living macrophages scanned with AFM tips coat- of the specimen (Supplementary Fig. 1). This imaging mode has ed with CD40 ligand (Supplementary Methods). been used since http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Methods Springer Journals

OpenFovea: open-source AFM data processing software

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References (6)

Publisher
Springer Journals
Copyright
Copyright © 2012 by Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
Subject
Life Sciences; Life Sciences, general; Biological Techniques; Biological Microscopy; Biomedical Engineering/Biotechnology; Bioinformatics; Proteomics
ISSN
1548-7091
eISSN
1548-7105
DOI
10.1038/nmeth.2112
Publisher site
See Article on Publisher Site

Abstract

No. not event correspond ence and Asylum Research (.ibw files). Support for Park Systems micro- OpenFovea: open-source AFM data scopes is under development. Images and graphs generated using OpenFovea can be saved as processing software bitmaps (.jpg, .png) or as vector images (.pdf, .svg). Raw data can To the Editor: The atomic force microscope (AFM) is often used be exported as comma-separated values that can be imported into to detect specific molecules on the surfaces of specimens and to spreadsheets or statistical software. OpenFovea is capable of saving measure a specimen’s mechanical properties . This information can the original force-distance curves as well as the computed param- be deduced from the force-distance curves generated by the deflec- eters and results. tion of the cantilever as it approaches, indents and recedes from To demonstrate the processing and display capabilities of our soft- the sample. Force-volume images can be generated from a series of ware, we used OpenFovea to process data collected in force-volume successive force-distance curves recorded while scanning an area imaging mode on living macrophages scanned with AFM tips coat- of the specimen (Supplementary Fig. 1). This imaging mode has ed with CD40 ligand (Supplementary Methods). been used since

Journal

Nature Methods – Springer Journals

Published: Jul 30, 2012

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