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Current protocols in molecular biology
- Publisher
- Wiley
- Copyright
- Copyright © 2001 Wiley‐Liss, Inc.
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- DOI
- 10.1002/dvdy.1132
- pmid
- 11376490
- Publisher site
- See Article on Publisher Site
Abstract
Zebrafish E‐cadherin (cdh1) cell adhesion molecule cDNAs were cloned. We investigated spatial and temporal expression of cdh1 during early embryogenesis. Expression was observed in blastomeres, the anterior mesoderm during gastrulation, and developing epithelial structures. In the developing nervous system, cdh1 was detected at the pharyngula stage (24 hpf) in the midbrain‐hindbrain boundary (MHB). Developmental regulation of MHB formation involves wnt1 and pax2.1. wnt1 expression preceded cdh1 expression during MHB formation, and cdh1 expression in the MHB was dependent on normal development of this structure. © 2001 Wiley‐Liss, Inc.
Journal
Developmental Dynamics – Wiley
Published: Jun 1, 2001