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Regulation of gibberellin 20-oxidase1 expression in spinach by photoperiod

Regulation of gibberellin 20-oxidase1 expression in spinach by photoperiod The multifunctional gibberellin (GA) 20-oxidase [GA53, 2-oxoglutarate:oxygen oxidoreductase (20-oxidizing), EC 1.14.11] has been extensively investigated in various species at the genetic and molecular levels, but not at the protein level. Here, we report on expression of GA20ox1 protein in spinach (Spinacia oleracea L.) in response to photoperiod. Polyclonal antibodies were raised against recombinant SoGA20ox1 in a chicken. These antibodies immuno-inhibited the enzymatic activity of the recombinant SoGA20ox1 and immuno-precipitated SoGA20ox1 (43 kDa) isolated from spinach shoot tips. Northern and western analyses showed that the levels of SoGA20ox1 transcript and protein increased in the blades, petioles, young leaves, and tips in response to long-day (LD) conditions. The transcript and protein levels of the SoGA20ox1 gene were up-regulated in the petioles and tips in a time-dependent manner. The estimated number of SoGA20ox1 protein molecules per cell was approximately 13-fold higher in tips grown in LD than in short-day (SD) conditions. The levels of SoGA20ox1 protein gradually decreased in tips when spinach plants grown in LD were transferred to SD conditions. SoGA20ox1 transcripts were detected by in situ hybridization in rapidly growing tissues—such as the shoot apical meristem, leaf and flower primordia, leaflets, and vascular tissues—but not in the expanding subapical region. In petioles, expression of SoGA20ox1 was detected in the companion cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Planta Springer Journals

Regulation of gibberellin 20-oxidase1 expression in spinach by photoperiod

Lee, Dong; Zeevaart, Jan
Planta , Volume 226 (1) – Jan 11, 2007
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References (38)

Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Life Sciences; Forestry; Ecology; Agriculture; Plant Sciences
ISSN
0032-0935
eISSN
1432-2048
DOI
10.1007/s00425-006-0463-1
pmid
17216482
Publisher site
See Article on Publisher Site

Abstract

The multifunctional gibberellin (GA) 20-oxidase [GA53, 2-oxoglutarate:oxygen oxidoreductase (20-oxidizing), EC 1.14.11] has been extensively investigated in various species at the genetic and molecular levels, but not at the protein level. Here, we report on expression of GA20ox1 protein in spinach (Spinacia oleracea L.) in response to photoperiod. Polyclonal antibodies were raised against recombinant SoGA20ox1 in a chicken. These antibodies immuno-inhibited the enzymatic activity of the recombinant SoGA20ox1 and immuno-precipitated SoGA20ox1 (43 kDa) isolated from spinach shoot tips. Northern and western analyses showed that the levels of SoGA20ox1 transcript and protein increased in the blades, petioles, young leaves, and tips in response to long-day (LD) conditions. The transcript and protein levels of the SoGA20ox1 gene were up-regulated in the petioles and tips in a time-dependent manner. The estimated number of SoGA20ox1 protein molecules per cell was approximately 13-fold higher in tips grown in LD than in short-day (SD) conditions. The levels of SoGA20ox1 protein gradually decreased in tips when spinach plants grown in LD were transferred to SD conditions. SoGA20ox1 transcripts were detected by in situ hybridization in rapidly growing tissues—such as the shoot apical meristem, leaf and flower primordia, leaflets, and vascular tissues—but not in the expanding subapical region. In petioles, expression of SoGA20ox1 was detected in the companion cells.

Journal

Planta – Springer Journals

Published: Jan 11, 2007

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