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Resting and Action Potentials of Nonischemic and Chronically Ischemic Human Ventricular Muscle

Resting and Action Potentials of Nonischemic and Chronically Ischemic Human Ventricular Muscle Membrane Potentials in Human Ventricle. Introduction: The effect of chronic ischemia on the electrical properties of human cardiac tissue is not well understood. Methods and Results: Membrane potentials were studied using microelectrode techniques in isolated human ventricular tissues obtained from nonischemic (n = 17) or chronically ischemic (n = 71 myocardium. In normal Tyrode's solution, resting potential (Vr) was lower in ischemic (−70.1 · 2.12 mV) than in nonischemic muscles (−77.6 · 0.93 mV; mean · SKM; P < 0.051. In high (K)0 (> 10 mM) media, V, was of similar magnitude in both types of tissue (in 21.6 mM (K)0 Vr was −53.1 · 2.24 mV in nonischcmic and −49.6 · 2.03 mV in ischemic preparations; n = 7 each; P > 0.05). Lowering (K)0. caused persistent Hyperpolarization in nonischemic muscles, but caused depolarization in chronically ischemic preparations (in 2,7 mM (K)0 Vr was −84.9 · 2.74 mV and –61.7 · 7.72 mV, respectively; n = 7; P < 0.05). Pinacidil (100 μM) normalized the response of chronically ischemic preparations to (K)0. Action potentials (APs) from nonischemic tissues varied in shape and could show aberrations. Kpinephrine (1.5 μM) and 4‐aminopyridine (3 mM) increased the AP duration, while butanedione monoxime (20 mM) and tetrodotoxin (1 μM) shortened it. In chronically ischemic muscles, the AP was characterized by the absence of a plateau and the presence of a slow phase of final repolarization. Conclusion: The differential effect of tow (K)0 on the resting membrane potential of nonischemic and chronically ischemic tissues suggests a change in the properties or the regulation of background K+ channels during chronic ischemia. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cardiovascular Electrophysiology Wiley

Resting and Action Potentials of Nonischemic and Chronically Ischemic Human Ventricular Muscle

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References (45)

Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1045-3873
eISSN
1540-8167
DOI
10.1111/j.1540-8167.1994.tb01189.x
Publisher site
See Article on Publisher Site

Abstract

Membrane Potentials in Human Ventricle. Introduction: The effect of chronic ischemia on the electrical properties of human cardiac tissue is not well understood. Methods and Results: Membrane potentials were studied using microelectrode techniques in isolated human ventricular tissues obtained from nonischemic (n = 17) or chronically ischemic (n = 71 myocardium. In normal Tyrode's solution, resting potential (Vr) was lower in ischemic (−70.1 · 2.12 mV) than in nonischemic muscles (−77.6 · 0.93 mV; mean · SKM; P < 0.051. In high (K)0 (> 10 mM) media, V, was of similar magnitude in both types of tissue (in 21.6 mM (K)0 Vr was −53.1 · 2.24 mV in nonischcmic and −49.6 · 2.03 mV in ischemic preparations; n = 7 each; P > 0.05). Lowering (K)0. caused persistent Hyperpolarization in nonischemic muscles, but caused depolarization in chronically ischemic preparations (in 2,7 mM (K)0 Vr was −84.9 · 2.74 mV and –61.7 · 7.72 mV, respectively; n = 7; P < 0.05). Pinacidil (100 μM) normalized the response of chronically ischemic preparations to (K)0. Action potentials (APs) from nonischemic tissues varied in shape and could show aberrations. Kpinephrine (1.5 μM) and 4‐aminopyridine (3 mM) increased the AP duration, while butanedione monoxime (20 mM) and tetrodotoxin (1 μM) shortened it. In chronically ischemic muscles, the AP was characterized by the absence of a plateau and the presence of a slow phase of final repolarization. Conclusion: The differential effect of tow (K)0 on the resting membrane potential of nonischemic and chronically ischemic tissues suggests a change in the properties or the regulation of background K+ channels during chronic ischemia.

Journal

Journal of Cardiovascular Electrophysiology – Wiley

Published: Aug 1, 1994

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