/lp/oxford-university-press/high-level-tuber-expression-and-sucrose-inducibility-of-a-potato-sus4-I6JbWtfqeB
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- Publisher
- Oxford University Press
- Copyright
- Copyright © 2021 American Society of Plant Biologists
- ISSN
- 1040-4651
- eISSN
- 1532-298X
- DOI
- 10.1105/tpc.7.9.1387
- Publisher site
- See Article on Publisher Site
Abstract
Abstract The 3.6 kb of 5' flanking sequence, leader intron, and 0.7 kb of 3' sequence from the potato sucrose synthase gene Sus4-16 are sufficient to direct high-level expression in developing tubers, in basal tissues of axillary buds and shoots, and in meristems and caps of roots, and to confer sucrose inducibility in leaves. By examining a series of deletion and substitution constructs in transgenic potato plants, we found that this pattern of expression requires 5' flanking sequences both upstream and downstream of position -1500 and that sequences between positions -1500 and -267 are essential for sucrose induction. Replacement of the native 3' sequence with the nopaline synthase 3' sequence resulted in the loss of sucrose inducibility and of expression in basal tissues of axillary buds. A general decrease in expression in other tissues was also observed. Removal of the 1612-bp leader intron also had a dramatic effect on both the pattern and level of expression. This content is only available as a PDF. © 1995 by American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Journal
The Plant Cell – Oxford University Press
Published: Sep 1, 1995