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Vitronectin is a critical protein adhesion substrate for IL‐4‐induced foreign body giant cell formation

Vitronectin is a critical protein adhesion substrate for IL‐4‐induced foreign body giant cell... An in vitro system of interleukin (IL)‐4‐induced foreign body giant cell (FBGC) formation was utilized to define the adhesion protein substrate(s) that promotes this aspect of the foreign body reaction on biomedical polymers. Human monocytes were cultured on cell culture polystyrene surfaces that had been pre‐adsorbed with a synthetic arginine‐glycine‐aspartate peptide previously found to support optimal FBGC formation, or with various concentrations of potential physiological protein substrates, i.e. complement C3bi, collagen types I or IV, fibrinogen, plasma fibronectin, fibroblast fibronectin, laminin, thrombospondin, vitronectin, or von Willebrand factor. Cultures were evaluated on days 0 (1.5 h), 3, and 7 by May–Grünwald/Giemsa staining. Initial monocyte adhesion occurred on all adsorbed proteins. However, by day 7 of culture, only vitronectin was striking in its ability to support significant macrophage adhesion, development, and fusion leading to FBGC formation. Vitronectin supported high degrees of FBGC formation at an absorption concentration between 5 and 25 μg/mL. These findings suggest that adsorbed vitronectin is critical in the collective events that support and promote FBGC formation on biomedical polymers, and that the propensity for vitronectin adsorption may underlie the material surface chemistry dependency of FBGC formation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Vitronectin is a critical protein adhesion substrate for IL‐4‐induced foreign body giant cell formation

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References (51)

Publisher
Wiley
Copyright
"Copyright © 2008 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.a.31658
pmid
17994558
Publisher site
See Article on Publisher Site

Abstract

An in vitro system of interleukin (IL)‐4‐induced foreign body giant cell (FBGC) formation was utilized to define the adhesion protein substrate(s) that promotes this aspect of the foreign body reaction on biomedical polymers. Human monocytes were cultured on cell culture polystyrene surfaces that had been pre‐adsorbed with a synthetic arginine‐glycine‐aspartate peptide previously found to support optimal FBGC formation, or with various concentrations of potential physiological protein substrates, i.e. complement C3bi, collagen types I or IV, fibrinogen, plasma fibronectin, fibroblast fibronectin, laminin, thrombospondin, vitronectin, or von Willebrand factor. Cultures were evaluated on days 0 (1.5 h), 3, and 7 by May–Grünwald/Giemsa staining. Initial monocyte adhesion occurred on all adsorbed proteins. However, by day 7 of culture, only vitronectin was striking in its ability to support significant macrophage adhesion, development, and fusion leading to FBGC formation. Vitronectin supported high degrees of FBGC formation at an absorption concentration between 5 and 25 μg/mL. These findings suggest that adsorbed vitronectin is critical in the collective events that support and promote FBGC formation on biomedical polymers, and that the propensity for vitronectin adsorption may underlie the material surface chemistry dependency of FBGC formation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

Journal

Journal of Biomedical Materials Research Part A – Wiley

Published: Aug 1, 2008

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