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The Peritoneal Fluid Levels of Interleukin‐12 in Women with Endometriosis

The Peritoneal Fluid Levels of Interleukin‐12 in Women with Endometriosis PROBLEM: Interleukin‐12 (IL‐12) is produced mainly by monocytes/macrophages, and it induces proliferation and cytotoxicity of T‐cells and natural killer cells. In women with endometriosis, natural killer cell activity in the peritoneal fluid is significantly decreased. We aimed to measure the peritoneal fluid level of IL‐12 in endometriosis. METHOD OF STUDY: We measured IL‐12 levels in peritoneal fluid samples from women with or without endometriosis and in supernatants from endometrial stromal, ovarian stromal, and mesothelial cell cultures, using a high‐sensitivity enzyme‐linked immunosorbent assay. RESULTS: The median concentration of IL‐12 in the peritoneal fluid of women with endometriosis was 1.1 pg/ml (range, 0.2–5.5) and was 1.6 pg/ml (range, 0.4‐2.8) in women without endometriosis, not a statistically significant difference. IL‐12 was not detected in the supernatants of endometrial stromal, ovarian stromal, and mesothelial cell cultures. CONCLUSION: Concentrations of IL‐12 in the peritoneal fluid of women with or without endometriosis are low, but they are detectable and are not affected significantly by the presence of endometriosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Reproductive Immunology Wiley

The Peritoneal Fluid Levels of Interleukin‐12 in Women with Endometriosis

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References (37)

Publisher
Wiley
Copyright
1998 Munksgaard
ISSN
1046-7408
eISSN
1600-0897
DOI
10.1111/j.1600-0897.1998.tb00347.x
Publisher site
See Article on Publisher Site

Abstract

PROBLEM: Interleukin‐12 (IL‐12) is produced mainly by monocytes/macrophages, and it induces proliferation and cytotoxicity of T‐cells and natural killer cells. In women with endometriosis, natural killer cell activity in the peritoneal fluid is significantly decreased. We aimed to measure the peritoneal fluid level of IL‐12 in endometriosis. METHOD OF STUDY: We measured IL‐12 levels in peritoneal fluid samples from women with or without endometriosis and in supernatants from endometrial stromal, ovarian stromal, and mesothelial cell cultures, using a high‐sensitivity enzyme‐linked immunosorbent assay. RESULTS: The median concentration of IL‐12 in the peritoneal fluid of women with endometriosis was 1.1 pg/ml (range, 0.2–5.5) and was 1.6 pg/ml (range, 0.4‐2.8) in women without endometriosis, not a statistically significant difference. IL‐12 was not detected in the supernatants of endometrial stromal, ovarian stromal, and mesothelial cell cultures. CONCLUSION: Concentrations of IL‐12 in the peritoneal fluid of women with or without endometriosis are low, but they are detectable and are not affected significantly by the presence of endometriosis.

Journal

American Journal of Reproductive ImmunologyWiley

Published: Feb 1, 1998

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