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Uptake and metabolism of radioactive gibberellins by barley aleurone layers

Uptake and metabolism of radioactive gibberellins by barley aleurone layers When aleurone layers were treated with labeled gibberellin A1 (3H-GA1), gibberellin A5 (3H-GA5) and the methyl ester of 3H-GA5 (3H-GA5-ME), radioactivity was accumulated by the tissue for a period of 20–30 h. After this time, radioactivity was released into the medium. Concomitantly, ribonuclease was also liberated by the tissue. The radioactivity accumulated by aleurone layers was associated with polar metabolites of the respective GAs, and the extent of extent of accumulation was a function of the degree of GA metabolism (GA5-ME>GA5>GA1). Accumulation of radioactivity was inhibited in the cold and by the metabolic poisons NaF and dinitrophenol. This was thought to be due to inbition of GA metabolism. The accumulation of 3H-GA1 in aleurone tissue did not reach saturation when unlabeled GA3 up to 10-2 M was added to the incubation medium. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Planta Springer Journals

Uptake and metabolism of radioactive gibberellins by barley aleurone layers

Musgrave, Alan; Kays, Sandra; Kende, Hans
Planta , Volume 102 (1) – Sep 14, 2004
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References (9)

Publisher
Springer Journals
Copyright
Copyright © 1972 by Springer-Verlag
Subject
Life Sciences; Plant Sciences; Agriculture; Ecology; Forestry
ISSN
0032-0935
eISSN
1432-2048
DOI
10.1007/BF00391445
Publisher site
See Article on Publisher Site

Abstract

When aleurone layers were treated with labeled gibberellin A1 (3H-GA1), gibberellin A5 (3H-GA5) and the methyl ester of 3H-GA5 (3H-GA5-ME), radioactivity was accumulated by the tissue for a period of 20–30 h. After this time, radioactivity was released into the medium. Concomitantly, ribonuclease was also liberated by the tissue. The radioactivity accumulated by aleurone layers was associated with polar metabolites of the respective GAs, and the extent of extent of accumulation was a function of the degree of GA metabolism (GA5-ME>GA5>GA1). Accumulation of radioactivity was inhibited in the cold and by the metabolic poisons NaF and dinitrophenol. This was thought to be due to inbition of GA metabolism. The accumulation of 3H-GA1 in aleurone tissue did not reach saturation when unlabeled GA3 up to 10-2 M was added to the incubation medium.

Journal

Planta – Springer Journals

Published: Sep 14, 2004

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